Molecular cytotaxonomy of the Triatoma brasiliensis species subcomplex (Hemiptera, Triatominae)

Author(s)

Chaboli Alevi, Kaio Cesar ^[1] ; Bittinelli, Isadora de Freitas ^{[1, 2]} ; Grzyb Delgado, Luiza Maria ^{[1, 2]} ; Madeira, Fernanda Fernandez ^[2] ; de Oliveira, Jader ^[1] ; Lilioso, Mauricio ^[3] ; Folly-Ramos, Elaine ^[4] ; da Rosa, Joao Aristeu ^[1] ; Vilela Azeredo-Oliveira, Maria Tercilia ^[2]

Affiliation

^[1] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, FCFAR UNESP, Lab Parasitol, Rodovia Araraquara Jau Km 1, BR-14801902 Araraquara, SP - Brazil

^[2] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, IBILCE UNESP, Lab Biol Celular, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, UNICAMP, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

^[4] Univ Fed Paraiba, Ctr Ciencias Aplicadas & Educ, Dept Engn & Meio Ambiente, UFPB, Lab Ecol Anim, Av Santa Elizabete 160, BR-58297000 Rio Tinto, PB - Brazil

• Document type: Journal article
• Source: Acta Tropica; v. 201, JAN 2020.
• Web of Science Citations: 1

Abstract

The Triatoma genus is paraphyletic, and its species are grouped into complexes and subcomplexes. Given the fact that species that make up a given subcomplex generally share chromosomal traits, we analyzed the distribution of AT- and CG-rich DNA of the T. brasiliensis species subcomplex, in order to establish affinities among members of the T. brasiliensis subcomplex based on chromatin and chromosome traits and develop an identification key for the four monophyletic Triatoma subcomplexes from South America. All species exhibited a CG-rich X sex chromosome and autosomes, as well as an AT-rich Y sex chromosome. This feature can be used as a diagnostic characteristic to determine whether a given species is a member of the T. brasiliensis subcomplex, because it enables the differentiation of these species from all Triatoma of South America. Thus, we confirmed the chromosomal relationship of the T. brasiliensis species subcomplex and developed a dichotomous key based on the chromocenter to differentiate the species from this subcomplex from the other monophyletic Triatoma subcomplexes from South America. (AU)

• FAPESP's process: 13/19764-0 - Cytotaxonomy and chromosomal evolution in Triatominae subfamily
• Grantee: Kaio Cesar Chaboli Alevi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/21359-8 - Integrated molecular approaches to elucidate the ecological associations of Triatoma brasiliensis: food sources, microbiota and diversity of Trypanosoma cruzi
• Grantee: Maurício Lilioso de Lucena Filho
• Support Opportunities: Scholarships in Brazil - Doctorate