Melatonin Action on the Activity of Phagocytes from the Colostrum of Obese Women

Author(s)

Morais, Tassiane C. ^{[1, 2]} ; Honorio-Franca, Adenilda C. ^[3] ; Fujimori, Mahmi ^[3] ; de Quental, Ocilma B. ^{[1, 4]} ; Pessoa, Rafael S. ^[3] ; Franca, Eduardo L. ^[3] ; de Abreu, Luiz C. ^{[5, 1, 2, 6]}

Affiliation

^[1] Ctr Univ Saude ABC, Lab Delineamento Estudos & Escrita Cient, BR-09060870 Santo Andre, SP - Brazil

^[2] Univ Sao Paulo, Fac Saude Publ, Postgrad Program Publ Hlth, BR-01246904 Sao Paulo, SP - Brazil

^[3] Univ Fed Mato Grosso UFMT, Inst Biol & Hlth Sci, BR-78600000 Barra Do Garcas, Mato Grosso - Brazil

^[4] Fac Santa Maria, Dept Nursing, BR-58900000 Cajazeiras - Brazil

^[5] Univ Limerick, Grad Entry Med Sch, Limerick V94 T9PX - Ireland

^[6] Escola Super Ciencias Santa Casa de MisericOrdia, Programa Mestrado Polit PUbl & Desenvolvimento Lo, BR-29045402 Vitoria, ES - Brazil

• Document type: Journal article
• Source: MEDICINA-LITHUANIA; v. 55, n. 10 OCT 2019.
• Web of Science Citations: 0

Abstract

Background and objectives: Breastfeeding promotion is an important public health strategy for counter-balancing the negative effects of maternal overweight and obesity. Colostrum contains melatonin, which can attenuate the impacts of excessive maternal weight and boost the infant's immune system. Therefore, the objective of this study was to analyze the effects of melatonin on mononuclear (MN) phagocytes from the colostrum of women with pre-gestational obesity. Materials and Methods: Colostrum samples were collected postpartum from 100 women at a public hospital in SAo Paulo, Brazil. The donors were divided into two groups: the control group and the high body mass index (BMI) group. Melatonin levels in the colostrum were determined by an ELISA Kit, and the functional activity of MN cells was assessed using the phagocytosis assay by flow cytometry, and reactive oxygen species (ROS), intracellular calcium, and apoptosis were assessed by fluorimetry using a microplate reader. Results: The colostrum of mothers with pre-gestational high BMI exhibited higher melatonin levels (p < 0.05) and lower phagocytosis (p < 0.05) and ROS release (p < 0.05). Superoxide release was similar between the normal and high BMI groups (p > 0.05). Intracellular calcium release and apoptosis were also higher in the high BMI group (p < 0.05). Melatonin levels likely increased the phagocytosis rate and reduced intracellular calcium release and the apoptosis index (p < 0.05). Conclusions: The results suggest that melatonin is a possible mechanism for maternal-infant protection against obesity and restores the functional activity of colostrum phagocytes in obese mothers. (AU)

• FAPESP's process: 15/01051-3 - FUNCTIONAL ACTIVITY OF CELL MONONUCLEAR COLOSTRUM MOTHERS WITH OVERWEIGHT AND OBESITY AND THE ROLE OF AGENTS IMMUNOMODULATORS: ADIPONECTIN, LEPTIN AND MELATONIN
• Grantee: Tassiane Cristina Morais
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/19922-0 - Functional activity of mononuclear cells of colostrum of mothers with overweight and obesity and the role of immunomodulators agents: adiponectin, leptin and melatonin.
• Grantee: Luiz Carlos de Abreu
• Support Opportunities: Regular Research Grants