Determination of ring-substituted amphetamines through automated online hollow fiber liquid-phase microextraction-liquid chromatography

Author(s)

Rodriguez Cabal, Luis Felipe ^[1] ; Vargas Medina, Deyber Arley ^[1] ; Costa, Jose Luiz ^[2] ; Lancas, Fernando Mauro ^[1] ; Santos-Neto, Alvaro Jose ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

• Document type: Journal article
• Source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 411, n. 29, p. 7889-7897, NOV 2019.
• Web of Science Citations: 0

Abstract

The present paper describes an original method for the online preconcentration and analysis of ring-substituted amphetamines in urine samples, used on the integration of robot-assisted hollow fiber liquid-phase microextraction (HF-LPME), high-performance liquid chromatography (HPLC), and fluorescence detection (FLD). A lab-made autosampler, actuating a 100-mu L syringe and equipped with a three-way solenoid microvalve, allowed the acceptor phase to flow through and be withdrawn from the lumen fiber, enabling the automated online transference of the enriched acceptor phase for chromatographic analysis, through a six-port switching valve. The developed online HF-LPME-LC/FLD method demonstrated high analytical throughput and confidence, facilitating the efficient extraction and determination of the target analytes, with minimal solvent consumption and sample manipulation, in a straightforward way. Sample cleanup, analyte uptake, and analysis were carried out in 14.5 min. Under optimal conditions, automated online HF-LPME showed excellent linearity, precision, and trueness, obtaining intraday RSDs between 2.9 and 9.2% (n = 6) and interday RSDs between 5.3 and 9.3% (n = 6). Enrichment factors (EFs) ranged between 14.2 and 15.7, extraction recoveries (ERs) ranged between 17.7 and 19.5%, and the limits of detection (S/N = 3) were 2.0, 3.0, and 3.0 mu g L-1 for MDA, MDMA, and MDEA, respectively. The method proved to be an effortless, rapid, reliable, and environment-friendly approach for the determination of drug abuse in urine samples. (AU)

• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/00432-1 - The Toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by the analysis of hair and oral fluid samples
• Grantee: José Luiz da Costa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/21950-5 - Automation strategies for miniaturized sample preparation, case: automated online in syringe stirred SDME
• Grantee: Deyber Arley Vargas Medina
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 10/19910-9 - Development of green chemistry strategies on chromatography and application to the evaluation of antibiotics degradation in wastewaters by fixed-bed anaerobic bioreactors
• Grantee: Alvaro José dos Santos Neto
• Support Opportunities: Regular Research Grants