Somatic copy number alterations in pleomorphic adenoma and recurrent pleomorphic adenoma

Author(s)

Mariano, Fernanda Viviane ^[1] ; Fidalgo, Felipe ^[2] ; Maion Casarim, Andre Luis ^[3] ; Martins, Antonio Santos ^[3] ; Scarini, Joao Figueira ^[4] ; de Lima Souza, Reydson Alcides ^[4] ; Egal, Erika Said ^[1] ; Kowalski, Luiz Paulo ^[5] ; Victorino Krepischi, Ana Cristina ^[6] ; Altemani, Albina ^[1]

Affiliation

^[1] Univ Estadual Campinas, Fac Med Sci, Pathol Dept, Campinas, SP - Brazil

^[2] AC Camargo Canc Ctr, Int Res Ctr, Sao Paulo, SP - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Head & Neck Surg Dept, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Piracicaba Dent Sch, Oral Pathol Dept, Piracicaba, SP - Brazil

^[5] AC Camargo Canc Ctr, Head & Neck Surg Dept, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 129, n. 1, p. 59-64, JAN 2020.
• Web of Science Citations: 0

Abstract

Objective. As the genetic changes in recurrent pleomorphic adenoma (RPA) have not yet been investigated, the aim of this study was to assess the genomic profile of somatic copy number alteration in RPA and pleomorphic adenoma (PA) by using array comparative genomic hybridization (aCGH). Study Design. Four cases of RPA and 13 cases of PA were evaluated by using aCGH, using a 180 K platform. Data were analyzed by using Nexus Copy Number Discovery. Results. The RPA group rarely showed any copy number alteration, except for 1 case that exhibited losses in 5 p15.33 p15.1, 5 g13.1 q35.3 and 12 q12 813.11. The PA group also showed few copy number alterations, and the most frequent findings involved chromosomes 8: 8p21.3-p12 (gain), 8q12.1 (loss), 8p23.3-q24.3 (gain), and 8q12.1-q21.11 (gain). Genomic amplifications were revealed in the PA group, and the relevant affected genes were MAML2 and LIFR. Conclusions. PA and RPA exhibit few somatic copy number alterations and show a similar genomic profile on aCGH. (AU)

• FAPESP's process: 15/07304-0 - Study of genetic and metabolic changes of pleomorphic adenoma and carcinoma ex pleomorphic adenoma by exome, gene expression and immunohistochemistry
• Grantee: Fernanda Viviane Mariano Brum Corrêa
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/00831-0 - Study of genetic and metabolic changes of pleomorphic adenoma and carcinoma ex pleomorphic adenoma by exome, gene expression and immunohistochemistry
• Grantee: Fernanda Viviane Mariano Brum Corrêa
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 11/23204-5 - Genomic profile study of carcinoma ex-pleomorphic adenoma by array-comparative genomic hybridization
• Grantee: Albina Messias de Almeida Milani Altemani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/23366-5 - Genomic profile study of carcinoma ex-pleomorphic adenoma by array-comparative genomic hybridization
• Grantee: Fernanda Viviane Mariano Brum Corrêa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral