Characterization of progenitor/stem cell population from human dental socket and their multidifferentiation potential

Author(s)

Miguita, Lucyene ^{[1, 2]} ; Zindel Deboni, Maria Cristina ^[3] ; Sharpe, Paul ^[4] ; Mantesso, Andrea ^{[1, 5]}

Affiliation

^[1] Univ Sao Paulo FOUSP, Sch Dent, Dept Oral & Maxillofacial Pathol, Av Prof Lineu Prestes 2227, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Fed Minas Gerais, Sch Dent, Dept Oral Surg & Pathol, St Prof Moacir Gomes de Freitas 688, Belo Horizonte, MG - Brazil

^[3] Univ Sao Paulo FOUSP, Sch Dent, Dept Oral & Maxillofacial Surg, Discipline Oral Surg, Av Prof Lineu Prestes 2227, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

^[4] Kings Coll London, Dept Craniofacial Dev & Stem Cell Biol, Dent Inst, Guys Hosp, Floor 18, Guys Tower, London SE1 9RT - England

^[5] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, 1011 N Univ Ave, Ann Arbor, MI 48109 - USA

• Document type: Journal article
• Source: CELL AND TISSUE BANKING; v. 21, n. 1 DEC 2019.
• Web of Science Citations: 0

Abstract

Dental stem cells have many applications in medicine, dentistry and stem cell biology in general due to their easy accessibility and low morbidity. A common surgical manoeuvre after a tooth extraction is the dental socket curettage which is necessary to clean the alveolus and favour alveolar bone healing. This procedure can cause very low morbidity compared to bone marrow collection procedures and the collected material is normally discarded. In order to investigate if the tissue obtained by dental socket curettage after a tooth extraction was a feasible alternative source to isolate human stem cells, we isolated and characterized two different stem cell populations based on STRO-1 and CD146 expression. We were able to collect and grow cells from dental socket of vital and non-vital teeth. Both populations were proliferative, clonogenic and expressed STRO-1, CD146, CD90, NG2, PDGFR-beta, which are markers found in stem cells, presented in vitro multiline-differentiation into osteogenic, chondrogenic, and adipogenic tissue, and in vivo transplanted cells formed mineralized tissue. Interestingly, STRO-1(+) clonogenic cells presented better multidifferentiation than CD146(+) cells. Our results showed that mesenchymal stem cells can be isolated from the tiny tissue collected by dental socket curettage after vital and non-vital tooth extraction and suggest that STRO-1 is an important marker to be used to sort cells with multidifferentiation capacity. (AU)

• FAPESP's process: 09/00041-3 - Isolation and characterization of stem cells from the alveolar bone area
• Grantee: Andrea Mantesso Pobocik
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/06936-0 - Isolation and characterization of stem cells from the alveolar bone area
• Grantee: Lucyene Miguita Luiz
• Support Opportunities: Scholarships in Brazil - Doctorate