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In vitro analysis of citotoxic effects of solutions used in ocular prosthesis disinfection in cells of ocular conjunctiva

Grant number: 14/25714-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2015
Effective date (End): February 29, 2016
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Daniela Micheline dos Santos
Grantee:Letícia de Oliveira Gonçalves
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The control of biofilm formation is very important to maintain the health of anophthalmic cavity in patients wearing ocular prosthesis and it can be accomplished by daily cleaning and disinfection techniques, with different solutions. Although the cytotoxic effect of these solutions has been tested in different cells, there is no report about their effect on cells of anophthalmic socket. Thus, this study aims to evaluate the cytotoxic potential of different disinfectant solutions in the cells of human conjunctiva. For this, N1 white acrylic resin samples specific for artificial sclera will be made. Samples of the acrylic resin will be distributed into 6 groups according to solution used: I - No disinfection (immersed in saline solution); II - neutral soap; III - 4% chlorhexidine; IV - Efferdent; V - 1% Triclosan and VI - Citronella. The samples will be disinfected daily, for different periods: 1, 7, 15, 30, 60 and 90 days, according to the protocol of each disinfecting solution. After each period, cytotoxicity test will be performed using the MTT analysis, 24 hours after contact of the cells with the extract released by the sample disinfected. Data from trials will be submitted to normality tests, in order to determine whether they come or not from a normal distribution. If statistically significant difference is indicated, the appropriate probability test will be applied, completing the analysis.