Molecular typing of Campylobacter jejuni strains: comparison among four different techniques

Author(s)

Frazao, Miliane Rodrigues ^[1] ; de Souza, Roberto Antonio ^[1] ; Cazentini Medeiros, Marta Ines ^[2] ; Duque, Sheila da Silva ^[3] ; Cao, Guojie ^[4] ; Allard, Marc William ^[4] ; Falcao, Juliana Pfrimer ^[1]

Affiliation

^[1] Univ Sao Paulo, FCFRP, Dept Anal Clin Toxicol & Bromatol, Av Cafe S-N, Campus Univ USP, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Inst Adolfo Lutz Ribeirao Preto, Rua Minas 877, BR-14085410 Ribeirao Preto, SP - Brazil

^[3] Fiocruz MS, Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ - Brazil

^[4] US FDA, Div Microbiol, Off Regular Sci, Ctr Food Safety & Appl Nutr, 5001 Campus Dr, College Pk, MD 20740 - USA

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 51, n. 2 DEC 2019.
• Web of Science Citations: 1

Abstract

This study compared the ability of pulsed-field gel electrophoresis (PFGE), flaA small variable region (SVR) sequencing, analysis of the clustered regularly interspaced short palindromic repeats locus by high resolution melting analysis (CRISPR-HRMA), and multilocus sequence typing (MLST) for typing 111 Campylobacter jejuni strains isolated from diverse sources during 20 years in Brazil. For this, we used previous results obtained by PFGE and flaA-SVR sequencing from our research group and performed CRISPR-HRMA and MLST typing for the first time. Furthermore, the discrimination index (DI) of each method was accessed. The DI for PFGE, flaA-SVR sequencing, CRISPR-HRMA, and MLST was 0.980, 0.932, 0.868, and 0.931, respectively. By PFGE and flaA-SVR sequencing, some strains from clinical and non-clinical sources and from humans and animals presented >= 80% similarity. Similarly, some strains from different origins presented the same ST and CRISPR-HRMA types. In conclusion, despite the different DI values, all assays provided the same epidemiological information suggesting that a potential transmission may have occurred between C. jejuni from clinical and non-clinical sources and from animals and humans in Brazil. Furthermore it was demonstrated the suitability of PFGE that should be used preferably together with MLST and/or flaA-SVR sequencing for typing C. jejuni strains. (AU)

• FAPESP's process: 14/13029-0 - Molecular characterization of Campylobacter jejuni and Campylobacter coli strains of diverse origins
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants