Efficiency of methionine and cystine utilization by broiler chickens using stable isotopes

Author(s)

Pacheco, Leticia G. ^[1] ; Sakomura, Nilva K. ^[1] ; Suzuki, Rafael M. ^[1] ; Dorigam, Juliano C. P. ^[2] ; Viana, Gabriel S. ^[3] ; Leme, Bruno B. ^[1]

Affiliation

^[1] UNESP, Dept Anim Sci, Jaboticabal - Brazil

^[2] Evonik Nutr & Care Gmbh, Hanau - Germany

^[3] Nat Resources Inst Finland, Jokioinen - Finland

• Document type: Journal article
• Source: JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION; v. 104, n. 1 NOV 2019.
• Web of Science Citations: 0

Abstract

The aim of this study was to develop a method to determine the efficiency of utilization of Met and Cys using stable isotopes in order to reduce the number of sacrificed animals relative to the comparative slaughter technique. Met and Cys efficiencies were obtained separately and as total SAA values. Twenty-one 14- to 28-day-old broiler chickens were fed experimental diets containing different Met:Cys ratios (44:56, 50:50 and 56:44). Birds were given diets with daily supplements of L-(N-15) Met (60 mmol/kg) or L-(N-15(2)) Cys (35 mmol/kg) throughout the entire experimental period. Excreta were collected daily, and birds were euthanized at the end of the trial to collect feather-free bodies and feathers. Samples were analysed for N-15 and N-15-Met content. The utilization efficiency for Met, Cys and Met + Cys for feather-free bodies was 55%, 75%, and 60%, while the efficiencies for feathers were estimated at 96%, 77% and 84% respectively. (AU)

• FAPESP's process: 15/25717-0 - Efficiency and incorporation of nitrogenous compounds from L- (15N) methionine on the growth of broiler chickens
• Grantee: Letícia Graziele Pacheco
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/25761-4 - Modeling production and nutritional requirements of poultry and fish
• Grantee: Nilva Kazue Sakomura
• Support Opportunities: Research Projects - Thematic Grants