| Full text | |
| Author(s): Show less - |
Von Borowski, Rafael Gomes
[1, 2]
;
Barros, Muriel Primon
[1]
;
da Silva, Denise Brentan
[3, 4]
;
Lopes, Norberto Peporine
[3]
;
Zimmer, Karine Rigon
[5]
;
Staats, Charley Christian
[5]
;
de Oliveira, Cristiane Bernardes
[1]
;
Giudice, Emmanuel
[2]
;
Gillet, Reynald
[2]
;
Macedo, Alexandre Jose
[1, 5]
;
Baggio Gnoatto, Simone Cristina
[1]
;
Zimmer, Aline Rigon
[1]
Total Authors: 12
|
| Affiliation: | [1] Univ Fed Rio Grande do Sul, Fac Farm, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS - Brazil
[2] Univ Rennes, GDR, UMR6290, CNRS, F-35000 Rennes - France
[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, Av Do Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[4] Univ Fed Mato Grosso do Sul UFMS, CCBS, Lab Prod Nat & Espectrometria Massas LAPNEM, Cidade Univ, CP 549, BR-79070900 Campo Grande, MS - Brazil
[5] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Av Bento Goncalves 9500, Predios 43421-43431, BR-91501970 Porto Alegre, RS - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | International Journal of Pharmaceutics; v. 574, JAN 25 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Medical devices (indwelling) have greatly improved healthcare. Nevertheless, infections related to the use of these apparatuses continue to be a major clinical concern. Biofilms form on surfaces after bacterial adhesion, and they function as bacterial reservoirs and as resistance and tolerance factors against antibiotics and the host immune response. Technological strategies to control biofilms and bacterial adhesion, such as the use of surface coatings, are being explored more frequently, and natural peptides may promote their development. In this study, we purified and identified antibiofilm peptides from Capsicum baccatum (red pepper) using chromatography-tandem mass spectrometry, MALDI-MS, MS/MS and bioinformatics. These peptides strongly controlled biofilm formation by Staphylococcus epidermidis, the most prevalent pathogen in device-related infections, without any antibiotic activity. Furthermore, natural peptide-coated surfaces dislayed effective antiadhesive proprieties and showed no cytotoxic effects against different representative human cell lines. Finally, we determined the lead peptide predicted by Mascot and identified CSP37, which may be useful as a prime structure for the design of new antibiofilm agents. Together, these results shed light on natural Capsicum peptides as a possible antiadhesive coat to prevent medical device colonization. (AU) | |
| FAPESP's process: | 09/54098-6 - Acquisition of a mass spectrometer for the generation of images at the Mass Spectrometry Center of the University of São Paulo Ribeirão Preto School of Pharmaceutical Sciences: studies of molecular localization of biologically active substances |
| Grantee: | Norberto Peporine Lopes |
| Support Opportunities: | Multi-user Equipment Program |