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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Aerobic glycolysis is a metabolic requirement to maintain the M2-like polarization of tumor-associated macrophages

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Author(s):
de-Brito, Natalia M. [1] ; Duncan-Moretti, Julia [1] ; da-Costa, Hayandra C. [1] ; Saldanha-Gama, Roberta [1] ; Paula-Neto, Heitor A. [2] ; Dorighello, Gabriel G. [3] ; Simoes, Rafael L. [1] ; Barja-Fidalgo, Christina [1]
Total Authors: 8
Affiliation:
[1] Univ Estado Rio De Janeiro, IBRAG, Dept Biol Celular, Lab Farmacol Celular & Mol, Rio De Janeiro, RJ - Brazil
[2] Univ Fed Rio De Janeiro, Fac Farm, Lab Alvos Mol, Rio De Janeiro, RJ - Brazil
[3] Univ Estadual Campinas, Fac Ciencias Med, Lab Bioenerget, Campinas - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH; v. 1867, n. 2 FEB 2020.
Web of Science Citations: 0
Abstract

Macrophages (MO) are versatile cells, assuming distinct functional phenotypes depending on the activating stimulus and the microenvironment. The differential activation of macrophages is supported by profound intracellular metabolic changes, being well accepted that the M1/M(LPS + IFN-gamma) phenotype rely on aerobic glycolysis, while M2/M(IL-4) macrophages depend on oxidative metabolism. On the other hand, although tumor-associated macrophages (TAMs) are characterized by their high expression of M2/M(IL-4) markers, is currently unclear whether TAMs present the same oxidative metabolic profile of M2/M(IL-4) cells. Herein, we demonstrate for the first time that despite their high expression of M2/M(IL-4) markers, TAMs show high glycolytic activity, with high lactate secretion similar to the M1/M(LPS + IFN-gamma) phenotype. This activity seems to be essential for the M2 profile of TAMs, since the inhibition of glycolysis, but not the impairment of the oxidative phosphorylation or pentose phosphate pathway, diminished the expression of M2/M(IL-4) markers. These novel data indicate that TAMs, although are usually phenotyped as M2/M(IL-4)-like macrophages, they are metabolically distinct from these cells, being rather similar to M1/M(LPS + IFN-gamma) macrophages, depending on the glycolytic metabolism to support their profile and functions. (AU)

FAPESP's process: 17/17728-8 - Mitochondrial function and dysfunction: implications for aging and associated diseases
Grantee:Aníbal Eugênio Vercesi
Support Opportunities: Research Projects - Thematic Grants