| Full text | |
| Author(s): |
Okuda, Ligia Shimabukuro
[1]
;
Iborra, Rodrigo Tallada
[2, 1]
;
Ramos, Paula
[1]
;
Machado, Ubiratan Fabres
[3]
;
Correa-Giannella, Maria Lucia
[4, 5]
;
Pickford, Russell
[6]
;
Woods, Tom
[7]
;
Brimble, Margaret Anne
[7]
;
Rye, Kerry-Anne
[8]
;
Passarelli, Marisa
[4, 1]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Lab Lipides LIM 10, Sao Paulo - Brazil
[2] Univ Sao Judas Tadeu, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[4] Univ Nove de Julho, Programa Posgrad Med, Sao Paulo - Brazil
[5] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Lab Carboidratos & Radioimunoensaio LIM 18, Sao Paulo - Brazil
[6] Univ New South Wales, Bioanalyt Mass Spectrometry Facil, Sydney, NSW - Australia
[7] Univ Auckland, Sch Chem Sci, Auckland - New Zealand
[8] Univ New South Wales, Sch Med Sci, Lipid Res Grp, Sydney, NSW - Australia
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | Mediators of Inflammation; v. 2020, JAN 14 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
We addressed how advanced glycation (AGE) affects the ability of apoA-IV to impair inflammation and restore the expression of genes involved in cholesterol efflux in lipopolysaccharide- (LPS-) treated macrophages. Recombinant human apoA-IV was nonenzymatically glycated by incubation with glycolaldehyde (GAD), incubated with cholesterol-loaded bone marrow-derived macrophages (BMDMs), and then stimulated with LPS prior to measurement of proinflammatory cytokines by ELISA. Genes involved in cholesterol efflux were quantified by RT-qPCR, and cholesterol efflux was measured by liquid scintillation counting. Carboxymethyllysine (CML) and pyrraline (PYR) levels, determined by Liquid Chromatography-Mass Spectrometry (LC-MS/MS), were greater in AGE-modified apoA-IV (AGE-apoA-IV) compared to unmodified-apoA-IV. AGE-apoA-IV inhibited expression of interleukin 6 (Il6), TNF-alpha (Tnf), IL-1 beta (Il1b), toll-like receptor 4 (Tlr4), tumor necrosis factor receptor-associated factor 6 (Traf6), Janus kinase 2/signal transducer and activator of transcription 3 (Jak2/Stat3), nuclear factor kappa B (Nfkb), and AGE receptor 1 (Ddost) as well as IL-6 and TNF-alpha secretion. AGE-apoA-IV alone did not change cholesterol efflux or ABCA-1 levels but was unable to restore the LPS-induced reduction in expression of Abca1 and Abcg1. AGE-apoA-IV inhibited inflammation but lost its ability to counteract the LPS-induced changes in expression of genes involved in macrophage cholesterol efflux that may contribute to atherosclerosis. (AU) | |
| FAPESP's process: | 13/23392-1 - Anti-inflammatory function of apolipoprotein A-IV in diabetes mellitus and its effects on reverse cholesterol transport from macrophages: influence of modification by advanced glycation |
| Grantee: | Ligia Shimabukuro Okuda |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 16/15603-0 - Unraveling mechanisms of glycemic control and chronic complications of Diabetes mellitus: contributions to human health |
| Grantee: | Ubiratan Fabres Machado |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/21072-5 - Influence of the glycemic control and nephropathy stage on the reverse cholesterol transport in Diabetes mellitus: role of metabolic memory induced by advanced glycated albumin |
| Grantee: | Marisa Passarelli |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/02854-7 - Anti-inflammatory role of apolipoprotein A-IV in diabetes mellitus and its impact on macrophage reverse cholesterol transport: influence of advanced glycation |
| Grantee: | Ligia Shimabukuro Okuda |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/26256-1 - Intracellular trafficking of HDL receptor - ABCA-1 - in macrophages treated with advanced glycated albumin: influence of glycemic control in reverse cholesterol transport |
| Grantee: | Rodrigo Tallada Iborra |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |