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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Situ Metabolomics of the Honeybee Brain: The Metabolism of L-Arginine through the Polyamine Pathway in the Proboscis Extension Response (PER)

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Author(s):
Pratavieira, Marcel [1] ; da Silva Menegasso, Anally Ribeiro [1] ; Roat, Thaisa [1] ; Malaspina, Osmar [1] ; Palma, Mario Sergio [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo State, UNESP, Rio Claro - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF PROTEOME RESEARCH; v. 19, n. 2, p. 832-844, FEB 2020.
Web of Science Citations: 0
Abstract

The proboscis extension response (PER) reflex may be used to condition the pairing of an odor with sucrose, which is applied to the antennae, in experiments to induce learning, where the odor represents a conditioned stimulus, while sucrose represents an unconditioned stimulus. A series of studies have been conducted on honeybees, relating learning and memory acquisition/retrieval using the PER as a strategy for accessing their ability to exhibit an unconditioned stimulus; however, the major metabolic processes involved in the PER are not well known. Thus, the aim of this investigation is profiling the metabolome of the honeybee brain involved in the PER In this study, a semi quantitative approach of matrix-assisted laser desorption ionization (MALDI) mass spectral imaging (MSI) was used to profile the most abundant metabolites of the honeybee brain that support the PER It was reported that execution of the PER requires the metabolic transformations of arginine, ornithine, and lysine as substrates for the production of putrescine, cadaverine, spermine, spermidine, 1,3-diaminopropane, and gamma-aminobutyric acid (GABA). Considering the global metabolome of the brain of honeybee workers, the PER requires the consumption of large amounts of cadaverine and 1,3-diaminopropane, in parallel with the biosynthesis of high amounts of spermine, spermidine, and ornithine. To exhibit the PER, the brain of honeybee workers processes the conversion of L-arginine and L-lysine through the polyamine pathway, with different regional metabolomic profiles at the individual neuropil level. The outcomes of this study using this metabolic route as a reference are indicating that the antennal lobes and the calices (medial and lateral) were the most active brain regions for supporting the PER. (AU)

FAPESP's process: 16/16212-5 - Natural proteopeptides from the Brazilian fauna, flora and microbiota as potential models for the rational development of new drugs of therapeutic use: isolation, structure elucidation, chemical synthesis and functional activity assays
Grantee:Mario Sergio Palma
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants