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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Combination of Chemical and Neurotrophin Stimulation Modulates Neurotransmitter Receptor Expression and Activity in Transdifferentiating Human Adipose Stromal Cells

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Author(s):
Nery, Arthur A. [1] ; Pereira, Ricardo L. [1] ; Bassaneze, Vinicius [2] ; Nascimento, Isis C. [1] ; Sherman, Lauren S. [3] ; Rameshwar, Pranela [3] ; Lameu, Claudiana [1] ; Ulrich, Henning [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Heart Inst InCor, Sao Paulo - Brazil
[3] Rutgers Biomed & Hlth Sci, New Jersey Med Sch, Dept Med, Div Hematol Oncol, Newark, NJ - USA
Total Affiliations: 3
Document type: Journal article
Source: STEM CELL REVIEWS AND REPORTS; v. 15, n. 6, p. 851-863, DEC 2019.
Web of Science Citations: 1
Abstract

Adipose stromal cells are promising tools for clinical applications in regeneration therapies, due to their ease of isolation from tissue and its high yield; however, their ability to transdifferentiate into neural phenotypes is still a matter of controversy. Here, we show that combined chemical and neurotrophin stimulation resulted in neuron-like morphology and regulated expression and activity of several genes involved in neurogenesis and neurotransmission as well as ion currents mediated by NMDA and GABA receptors. Among them, expression patterns of genes coding for kinin-B1 and B2, alpha 7 nicotinic, M1, M3 and M4 muscarinic acetylcholine, glutamatergic (AMPA2 and mGlu2), purinergic P2Y1 and P2Y4 and GABAergic (GABA-A, beta 3-subunit) receptors and neuronal nitric oxide synthase were up-regulated compared to levels of undifferentiated cells. Simultaneously, expression levels of P2X1, P2X4, P2X7 and P2Y6 purinergic and M5 muscarinic acetylcholine receptors were down-regulated. Agonist-induced activity levels of the studied receptor classes also augmented during neuronal transdifferentiation. Transdifferentiated cells expressed high levels of neuronal beta 3-tubulin, NF-H, NeuN and MAP-2 proteins as well as increased ASCL1, MYT1 and POU3F2 gene expression known to drive neuronal fate determination. The presented work contributes to a better understanding of transdifferentiation induced by neurotrophins for a prospective broad spectrum of medical applications. (AU)

FAPESP's process: 12/50880-4 - Stem cells: from basic studies of kinin and purinergic receptor roles towards therapeutical applications
Grantee:Alexander Henning Ulrich
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/19128-2 - Metastasis mechanisms of childhood tumors to bone marrow
Grantee:Claudiana Lameu
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/18730-0 - Lung cancer stem cells by DNA aptamers identification / purification and kallikrein-kinin system study on tumor progression
Grantee:Isis Cristina Corrêa Do Nascimento
Support Opportunities: Scholarships in Brazil - Post-Doctoral