First report of cis-1,4-polyisoprene degradation by Gordonia paraffinivorans

Author(s):Show less -

Braga, Stefania Pegorin ^{[1, 2]} ; dos Santos, Alexandre Paes ^[2] ; Paganini, Thais ^[2] ; Barbosa, Deibs ^[3] ; Condomitti Epamino, George Willian ^[3] ; Morais, Carlos ^[3] ; Martins, Layla Farage ^[3] ; Silva, Aline Maria ^[3] ; Setubal, Joao Carlos ^{[3, 4]} ; Vallim, Marcelo Afonso ^[2] ; Pascon, Renata Castiglioni ^[2]

Affiliation

^[1] Univ Sao Paulo, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Diadema - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[4] Virginia Tech, Biocomplex Inst, Blacksburg, VA - USA

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 50, n. 4, p. 1051-1062, OCT 2019.
• Web of Science Citations: 0

Abstract

The use of rubber has increased over the years, leading to a series of environmental problems due to its indefinite decomposition time. Bioremediation employing microorganisms have drawn an increasing interest and originated several studies of microbial rubber degradation. Genome sequencing and in silico analysis demonstrated that G. paraffinivorans MTZ041 isolate encodes the lcp gene (Latex Clearing Protein), responsible for expressing an enzyme that performs the first step in the assimilation of synthetic and natural rubber. Growth curves and scanning electron microscopy (SEM) were conducted for MTZ041 in natural (NR) and synthetic rubber (IR) as sole carbon source during 11 weeks. After 80 days, robust growth was observed and SEM analysis revealed the presence of bacilli and the formation of biofilm-like structures on natural and synthetic rubber. This is the first report of a G. paraffinivorans rubber degrader. Given the complexity of this substrate and the relative small number of microorganisms with this ability, the description and characterization of MTZ041 is of great importance on bioremediation processes of rubber products. (AU)

• FAPESP's process: 16/07360-0 - Evaluation of the transformation potential of toxic compounds by isolates Gordonia paraffinivorans and Gordonia sihwensis deriving from composting
• Grantee: Stefania Pegorin Braga
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/50870-6 - Studies of microbial diversity in the Zoological Park of the State of São Paulo
• Grantee: João Carlos Setubal
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 16/14542-8 - Genetic characterization of the regulatory elements of the biosynthesis and uptake of amino acids and their role in virulence of Cryptococcus neoformans.
• Grantee: Renata Castiglioni Pascon
• Support Opportunities: Regular Research Grants