A simple procedure to obtain nanodiamonds from leftover of HFCVD system for biological application

Author(s):Show less -

Hurtado, Carolina Ramos ^{[1, 2]} ; Wachesk, Cristiane da Costa ^{[1, 3]} ; Queiroz, Rafaela Campos ^{[1, 2]} ; de Macedo, Erenilda Ferreira ^[1] ; Borja de Oliveira Correia, Rebeca Falcao ^[3] ; Taiariol, Thalita Sani ^[3] ; Diniz, Milton Faria ^[4] ; Isaias dos Santos, Alexandre Martins ^[1] ; do Amaral Montanheiro, Thais Larissa ^[5] ; Hurtado, Gabriela Ramos ^[6] ; Trava-Airoldi, Vladimir Jesus ^[3] ; Tada, Dayane Batista ^[1]

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Nanomat & Nanotoxicol Lab, Talim 330, BR-12231280 Sao Jose Dos Campos, SP - Brazil

^[2] Fed Inst Sao Paulo IFSP, Rod Pres Dutra, Km 145, BR-12223201 Sao Jose Dos Campos, SP - Brazil

^[3] Natl Inst Space Res INPE, Sensors & Mat Associated Lab, Av Astronautas 1-758, BR-12227010 Sao Jose Dos Campos, SP - Brazil

^[4] Inst Aeronaut & Space IAE, Aerosp Sci & Technol Dept, Praca Marechal Eduardo Gomes 50, BR-12228900 Sao Jose Dos Campos, SP - Brazil

^[5] Technol Inst Aerocaut ITA, Fundamental Sci Div, Praca Marechal Eduardo Gomes 50, BR-12228900 Sao Jose Dos Campos, SP - Brazil

^[6] Sao Paulo State Univ UNESP, Inst Sci & Technol, Rod Pres Dutra, Km 137, 8 Eugenio Melo, BR-12247004 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: SN APPLIED SCIENCES; v. 2, n. 3 MAR 2020.
• Web of Science Citations: 0

Abstract

Nanodiamonds (NDs) are amongst the most investigated carbon-based nanostructures due to their chemical stability and favorable mechanical properties. Despite the number of works on methods for NDs production, one of the main challenges is to achieve their colloidal stability in aqueous suspension. Additionally, NDs are normally obtained by expensive, complex and time-consuming process. Herein, it was presented a facile method to obtain NDs in aqueous suspension by using columnar structure diamond from Hot-Filament Chemical Vapour Deposition reactor (HFCVD). CVD diamond leftover thick film from CVDVale Company was used. Therefore, this method has the advantage of being not only practical but also cost-effective since it brings a profitable use of CVD diamond leftover. The Diamond thick film was submitted to ultrasonic cavitation in the presence and absence of ZrO2 microbeads in aqueous medium. The NDs hydrodynamic diameter and the stability in aqueous suspension were monitored by light scattering, size and morphology were analyzed by transmission electronic microscopy. Considering the wide application of NDs in biomedical devices, cytotoxicity of aqueous suspensions of NDs was evaluated against murine embryonic fibroblast cells. Furthermore, NDs were functionalized with hydrogen and carboxyl groups. NDs aqueous suspension of straight size distribution was obtained even in the absence of ZrO2 beads, indicating that they may be dispensable in order to decrease NDs size. NDs of average hydrodynamic diameter of 22 nm and -35 mV of Zeta-potential were obtained after ultrasonic cavitation followed by 2 h of centrifugation, not demonstrating cytotoxicity to cells at very low (0.05-0.5 mu g/mL) nor at higher concentrations (116 mu g/mL). Nevertheless, NDs showed a moderate cytotoxicity at intermediary concentration range (0.5-2.2 mu g/mL). From our knowledge, this is the first work that reports on a facile method for providing NDs aqueous suspension with high colloidal stability from HFCVD diamond leftover. (AU)

• FAPESP's process: 12/15857-1 - Scientific studies and innovation application on CVD diamond, DLC and carbon nanostructures obtained by chemical vapor deposition technique
• Grantee: Vladimir Jesus Trava-Airoldi
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/01697-6 - Synthesis and functionalization of bimetallic nanoparticles for application in photodynamic therapy of cancer
• Grantee: Dayane Batista Tada
• Support Opportunities: Regular Research Grants