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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

SAXS-CT: a nanostructure resolving microscopy for macroscopic biologic specimens

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Author(s):
Conceicao, A. L. C. [1, 2] ; Perlich, J. [2, 3] ; Haas, S. [2] ; Funari, S. S. [2]
Total Authors: 4
Affiliation:
[1] Univ Tecnol Fed Parana, Av Sete Setembro 3165, BR-80230901 Curitiba, Parana - Brazil
[2] Deutsch Elektronen Synchrotron DESY, Notkestr 85, D-22607 Hamburg - Germany
[3] Continental Reifen Deutschland GmbH, Jadekamp 30, D-30419 Hannover - Germany
Total Affiliations: 3
Document type: Journal article
Source: BIOMEDICAL PHYSICS & ENGINEERING EXPRESS; v. 6, n. 3 MAR 2020.
Web of Science Citations: 0
Abstract

SAXS-CT is an emerging powerful imaging technique which bridges the gap between information retrieved from high-resolution local techniques and information from low-resolution, large field-of-view imaging, to determine the nanostructure characteristics of well-ordered tissues, e.g., mineralized collagen in bone. However, in the case of soft tissues, features such as poor nanostructural organization and high susceptibility to radiation-induced damage limit the use of SAXS-CT. Here, by combining the freeze-drying the specimen, preceded by formalin fixation, with the nanostructure survey we identified and monitored alterations on the hierarchical arrangement of triglycerides and collagen fibrils three-dimensionally in breast tumor specimens without requiring sample staining. A high density of aligned collagen was observed precisely on the invasion front of the breast carcinoma, showing the direction of cancer spread, whereas substantial content of triglycerides was identified, where the healthy tissue was located. Finally, the approach developed here provides a path to high-resolution nanostructural probing with a large field-of-view, which was demonstrated through the visualization of characteristic nanostructural arrangement and quantification of content and degree of organization of collagen fibrils in normal, benign and malignant human breast tissue. (AU)

FAPESP's process: 11/20632-6 - Study of application of X-ray scattering techniques to produce breast nanoimages
Grantee:André Luiz Coelho Conceição
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor