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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High Genomic Variability in Equine Infectious Anemia Virus Obtained from Naturally Infected Horses in Pantanal, Brazil: An Endemic Region Case

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Author(s):
Malossi, Camila Dantas [1] ; Fioratti, Eduardo Gorzoni [2] ; Cardoso, Jedson Ferreira [3] ; Magro, Angelo Jose [1] ; Kroon, Erna Geessien [4] ; Aguiar, Daniel Moura de [5] ; Borges, Alice Mamede Costa Marque [5] ; Nogueira, Marcia Furlan [6] ; Ullmann, Leila Sabrina [1] ; Araujo, Jr., Joao Pessoa [1]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ Unesp, Inst Biotechnol, BR-18607440 Botucatu, SP - Brazil
[2] Vales Jequitinhonha & Mucuri Fed Univ UFVJM, Agr Sci Inst ICA, BR-39803371 Unai - Brazil
[3] Evandro Chagas Inst, Ctr Technol Innovat, BR-67030000 Ananindeua - Brazil
[4] Univ Fed Minas Gerais, Virol Lab, BR-31270901 Belo Horizonte, MG - Brazil
[5] Univ Fed Mato Grosso, Virol & Rickettsiosis Lab, BR-78060900 Cuiaba - Brazil
[6] EMBRAPA Pantanal, BR-79320900 Corumba - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Viruses-Basel; v. 12, n. 2 FEB 2020.
Web of Science Citations: 1
Abstract

Equine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectious anemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory in this area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAV are currently available only for gag and LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic area in Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing (NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning, Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilian strain comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions. (AU)

FAPESP's process: 14/13532-3 - Genomic and transcriptomic studies of viral pathogens in animals
Grantee:João Pessoa Araújo Junior
Support Opportunities: Regular Research Grants