Systemic and local effects of radiotherapy: an experimental study on implants placed in rats

Author(s):Show less -

Vegian, Mariana Raquel da Cruz ^[1] ; Costa, Bruno Cesar Almeida ^[1] ; Santana-Melo, Gabriela de Fatima ^[1] ; Godoi, Fernanda Herrera Costa ^[1] ; Kaminagakura, Estela ^[1] ; Tango, Rubens Nisie ^[2] ; do Prado, Renata Falchete ^[1] ; de Oliveira, Luciane Dias ^[1] ; Federico, Claudio Antonio ^[3] ; Avelino, Sarah de Oliveira Marco ^[4] ; Neves, Rafael Marques ^[1] ; de Vasconcellos, Luana Marotta Reis ^[1]

Affiliation

^[1] Sao Paulo State Univ Unesp, Inst Sci & Technol Sao Jose dos Campos, Dept Biosci & Oral Diag, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Inst Sci & Technol Sao Jose dos Campos, Dept Dent Mat & Prosthodont, Sao Jose Dos Campos, SP - Brazil

^[3] Technol Inst Aeronaut, Inst Adv Studies, Dept Aerosp Sci & Technol, Sao Jose Dos Campos, SP - Brazil

^[4] Sao Paulo State Univ Unesp, Inst Sci & Technol Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 24, n. 2, p. 785-797, FEB 2020.
• Web of Science Citations: 0

Abstract

Objectives Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. Material and methods Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; late-irradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-\& x251;, IL-1 beta, TGF-beta, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test (p < 0.05) was applied. Results The results indicated that ionizing radiation modifies the production of pro- and anti-inflammatory serum cytokines, the expression of proteins involved in bone remodeling and cellular apoptosis, as well as changes in bone formation. Conclusions The results suggests that a longer period between radiotherapy and implant placement surgery when irradiation occurs prior to implant installation would allow the recovery and renewal of bone cells and avoid future failures in osseointegration. (AU)

• FAPESP's process: 17/04389-0 - Modulator effect of ionizing radiation on blood cytokines in rats submitted to implants surgery
• Grantee: Sarah de Oliveira Marco Avelino
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/00543-5 - Effects of radiotherapy in bone-implant interface and on the tissues surrounding
• Grantee: Rafael Marques Neves
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/19707-5 - Modulator effect of ionizing radiation on cytokines blood and tissue cells bone present at interface bone-implant
• Grantee: Mariana Raquel da Cruz Vegian
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/24986-8 - Effects of radiatherapy on new bone formation, osseointegration, normal surrounding tissues and mesenchymal cells
• Grantee: Luana Marotta Reis de Vasconcellos
• Support Opportunities: Regular Research Grants