Microbial community analyses by high-throughput sequencing of rumen microorganisms fermenting office paper in mesophilic and thermophilic lysimeters

Author(s)

Botta, Livia Silva ^[1] ; Delforno, Tiago Palladino ^[2] ; Silva Rabelo, Camila Abreu B. ^[1] ; Silva, Edson Luiz ^[3] ; Amancio Varesche, Maria Bernadete ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul & Sanitat, Lab Biol Proc, Av Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Estadual Campinas, Div Microbial Resources, Chem Biol & Agr Pluridisciplinary Res Ctr, BR-13083970 Campinas, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Chem Engn, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: PROCESS SAFETY AND ENVIRONMENTAL PROTECTION; v. 136, p. 182-193, APR 2020.
• Web of Science Citations: 0

Abstract

This study explored the hydrolysis and fermentation of paper with hydrogen (H-2) and volatile fatty acid (VFA) recovery using a natural microbial consortium added to lysimeters. Serial dilutions of rumen fluid in natura were performed to obtain a fermentative nonmethanogen consortium, which was used as inoculum in lysimeters under two different incubation temperatures, Ll at 35 degrees C and L2 at 55 degrees C. H-2 was detected in both lysimeters: 791.5 mL (L1) and 70.4 mL (L2). In L1, the production of 16,856 mg L-1 acetic acid was likely due to the occurrence of homoacetogenesis. Under thermophilic conditions, L2, a low volume of H2 was detected, and the highlight was the production of ethanol and methanol at 2289 and 5614 mg L-1, respectively. According to the Shannon index, there was great diversity of microorganism populations in the rumen fluid in natura (6.34) compared with the purified rumen fluid (2.73), L1 (2.67) and L2 (3.21). The most abundant genus in the rumen fluid in natura was Prevotella (37.5 %); in the purified consortium (68.7 %) and L1 (63.8), Dysgonomonas; and in L2 (88.7 %), Thermicanus. In conclusion, the temperature influenced the microbial community structure, the metabolic route and, consequently, the main byproducts produced by the fermentative activity at 35 degrees C and 55 degrees C. (C) 2020 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/04200-4 - Hydrogen Production from Paper in Lysimeter by Cellulolytic Fermentation
• Grantee: Lívia Silva Botta
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/26470-7 - Influence of sugarcane bagasse saccharification by laccase for fermentative production of biofuels
• Grantee: Camila Abreu Borges da Silva Rabelo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral