Effects of Microplastics Associated with Triclosan on the Oyster Crassostrea brasiliana: An Integrated Biomarker Approach

Author(s)

Nobre, Caio Rodrigues ^[1] ; Moreno, Beatriz Barbosa ^[2] ; Alves, Aline Vecchio ^[2] ; Rosa, Jonas de Lima ^[3] ; Franco, Heloisa da Rosa ^[3] ; de Souza Abessa, Denis Moledo ^[1] ; Maranho, Luciane Alves ^[1] ; Choueri, Rodrigo Brasil ^[2] ; Gusso-Choueri, Paloma Kachel ^{[3, 1]} ; Seabra Pereira, Camilo Dias ^{[3, 2]}

Affiliation

^[1] Sao Paulo State Univ, Biosci Inst, Sao Vicente, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Marine Sci, Santos, SP - Brazil

^[3] Univ Santa Cecilia, Dept Ecotoxicol, Santos, SP - Brazil

• Document type: Journal article
• Source: Archives of Environmental Contamination and Toxicology; v. 79, n. 1 APR 2020.
• Web of Science Citations: 0

Abstract

Urban waste is a complex mixture of different substances, including microplastics and pharmaceuticals and personal care products. Microplastics have a high affinity for hydrophobic substances. One of these substances is triclosan, a bactericide used in a variety of hygiene products. Therefore, microplastics (MPs) may serve as a vector between triclosan and aquatic organisms. The current study sought to evaluate the effects of the interaction between microplastics and triclosan based on a mechanistic approach in which the oyster Crassostrea brasiliana was used as a model. The organisms were exposed to three conditions: the control, microplastic (MP), and microplastic contaminated with triclosan (MPT). The organisms were exposed for 3 or 7 days. After the exposure time, hemolymph was sampled for performing the neutral red retention time assay and, subsequently, the gills, digestive glands, and adductor muscles were dissected for measuring biomarkers responses (EROD, DBF, GST, GPx, GSH, lipid peroxidation, DNA strand breaks, and AChE). Our results demonstrate combined effects of MPs associated with triclosan on oyster physiology and biochemistry, as well as on lysosomal membrane stability. These results contribute to understanding the effects of contaminants of emerging concern and microplastics on aquatic organisms. (AU)

• FAPESP's process: 17/12193-9 - EVALUATION OF THE ENVIRONMENTAL RISK OF THE INTERACTION OF MICROPLASTIC, DRUG AND ANTIMICROBIAL: AN ECOTOXICOLOGICAL APPROACH
• Grantee: Caio Rodrigues Nobre
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/04970-5 - Relationship between metal concentrations in the sediment, bioaccumulation and toxic responses in p. vivipara:subsidies for the environmental risk assessment
• Grantee: Paloma Kachel Gusso Choueri
• Support Opportunities: Scholarships in Brazil - Post-Doctoral