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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Vestitol drives LPS-activated macrophages into M2 phenotype through modulation of NF-kappa B pathway

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Bueno-Silva, Bruno [1, 2] ; Rosalen, Pedro L. [3] ; Alencar, Severino M. [4] ; Mayer, Marcia P. A. [1]
Total Authors: 4
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo, SP - Brazil
[2] Univ Guarulhos, Dent Res Div, BR-07023070 Guarulhos, SP - Brazil
[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, UNICAMP, POB 52, BR-13414903 Piracicaba, SP - Brazil
[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, POB 9, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: International Immunopharmacology; v. 82, MAY 2020.
Web of Science Citations: 0

Previously, we demonstrated the anti-inflammatory properties of vestitol in a neutrophil model. Here, we show the effects of vestitol on macrophage activation and function. Vestitol was obtained from Brazilian red propolis after bioguided fractionation and tested at different concentrations in LPS-activated RAW 264.7 murine mac- rophages for nitric oxide (NO) production and cell viability. The levels of TNF-alpha, IL1-beta, TGF-beta, IL-4, IL-6, IL-10, IL-12, GM-CSF, IFN-gamma and gene expression related to cytokines, NO, PI3K-AKT and signal transduction pathways were assayed by ELISA and RT-qPCR, respectively. Differences were determined by one-way ANOVA followed by Tukey-Kramer. Vestitol inhibited NO production by 83% at 0.55 mu M without affecting cell viability when compared to the vehicle control (P < 0.05). Treatment with vestitol reduced GM-CSF, IL-6, TNF-alpha, IL-4 and TGF-beta levels and increased IL-10 release (P < 0.05). Vestitol affected the expression of genes related to NF-kappa B pathway, NO synthase, and inhibition of leukocyte transmigration, namely: Ccs, Ccng1, Calm1, Tnfsf15, Il11, Gata3, Gadd45b, Cdkn1b, Csf1, Ccl5, Birc3 (negatively regulated), and Igf1 (positively regulated). Vestitol diminished the activation of NF-kappa B and Erk 1/2 pathways and induced macrophages into M2-like polarization. The modulatory effects of vestitol are due to inhibition of NF-kappa B and Erk 1/2 signaling pathways, which are associated with the production of pro-inflammatory factors. (AU)

FAPESP's process: 12/14323-3 - Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response
Grantee:Marcia Pinto Alves Mayer
Support type: Regular Research Grants
FAPESP's process: 12/01500-4 - Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response
Grantee:Bruno Bueno Silva
Support type: Scholarships in Brazil - Post-Doctorate