Ligninolytic Enzymes of Endospore-Forming Bacillus aryabhattai BA03

Author(s)

Paz, Alicia ^[1] ; Costa-Trigo, Ivan ^[1] ; Oliveira, Ricardo Pinheiro de Souza ^[2] ; Dominguez, Jose Manuel ^[1]

Affiliation

^[1] Univ Vigo, Chem Engn Dept, Ind Biotechnol & Environm Engn Grp BiotecnIA, Campus Ourense, Lagoas S-N, Orense 32004 - Spain

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Av Lineu Prestes 580, Bl 16, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Current Microbiology; v. 77, n. 5, p. 702-709, MAY 2020.
• Web of Science Citations: 0

Abstract

In this work we investigated the enzymes produced by Bacillus aryabhattai BA03, particularly in those involved in ligninolytic activities such as laccases (Lac), lignin peroxidases (LiP) and Mn-dependent peroxidase (MnD-P). In this way, the maximal production of Lac (0.069 +/- 0.000 U/mL) was obtained at pH 9, and 37 oC after 72 h. LiP expressed the highest activity at 96 h in acid medium at 37 oC (0.741 +/- 0.029 U/mL). Meanwhile, the strain produced MnD-P (1.052 +/- 0.001 U/mL) at the highest temperature assayed (44 oC) and pH 7 at 72 h. In addition, this microorganism produced resistant endospores able to germinate after the sterilization program (121 oC, 15 min) showing a high enzymatic activity. Using the heat-treated culture as inoculum, the percentage of decolorization of 150 mg/L of Coomassie Brillant Blue reached 89.42 +/- 0.11% in only 24 h. These results open the use of these enzymes and endospores in bioremediation processes carried out under different temperatures and pH values. (AU)

• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants