Insulin does not stimulate beta-alanine transport into human skeletal muscle

Author(s):Show less -

Goncalves, Livia de Souza ^{[1, 2]} ; Kratz, Caroline ^{[1, 2]} ; Santos, Livia ^[3] ; Carvalho, Victor Henrique ^[4] ; Sales, Lucas Peixoto ^{[1, 2]} ; Nemezio, Kleiner ^{[1, 2]} ; Longobardi, Igor ^{[1, 2]} ; Riani, Luiz Augusto ^{[1, 2]} ; de Oliveira Lima, Marcelo Miranda ^[5] ; Saito, Tiemi ^{[1, 2]} ; Fernandes, Alan Lins ^{[1, 2]} ; Rodrigues, Joice ^[4] ; James, Ruth Margaret ^[3] ; Sale, Craig ^[3] ; Gualano, Bruno ^{[1, 2]} ; Geloneze, Bruno ^[5] ; Gennari de Medeiros, Marisa Helena ^[4] ; Artioli, Guilherme Giannini ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med FMUSP, Appl Physiol & Nutr Res Grp, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med FMUSP, Sch Phys Educ & Sport, Rheumatol Div, Sao Paulo - Brazil

^[3] Nottingham Trent Univ, Musculoskeletal Physiol Res Grp, Sport Hlth & Performance Enhancement Res Ctr, Nottingham - England

^[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[5] Univ Estadual Campinas, UNICAMP, Lab Invest Metab & Diabet LIMED, Gastroctr, Dept Cirurgia, Campinas, SP - Brazil

• Document type: Journal article
• Source: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY; v. 318, n. 4, p. C777-C786, APR 2020.
• Web of Science Citations: 0

Abstract

To test whether high circulating insulin concentrations influence the transport of beta-alanine into skeletal muscle at either saturating or subsaturating beta-alanine concentrations, we conducted two experiments whereby beta-alanine and insulin concentrations were controlled. In experiment 1, 12 men received supraphysiological amounts of beta-alanine intravenously (0.11 g.kg(-1).min(-1) for 150 min), with or without insulin infusion. beta-Alanine and carnosine were measured in muscle before and 30 min after infusion. Blood samples were taken throughout the infusion protocol for plasma insulin and beta-alanine analyses. beta-Alanine content in 24-h urine was assessed. In experiment 2, six men ingested typical doses of beta-alanine (10 mg/kg) before insulin infusion or no infusion. beta-Alanine was assessed in muscle before and 120 min following ingestion. In experiment 1, no differences between conditions were shown for plasma beta-alanine, muscle beta-alanine, muscle carnosine and urinary beta-alanine concentrations (all P > 0.05). In experiment 2, no differences between conditions were shown for plasma beta-alanine or muscle beta-alanine concentrations (all P > 0.05). Hyperinsulinemia did not increase beta-alanine uptake by skeletal muscle cells, neither when substrate concentrations exceed the V-max of beta-alanine transporter TauT nor when it was below saturation. These results suggest that increasing insulin concentration is not necessary to maximize beta-alanine transport into muscle following beta-alanine intake. (AU)

• FAPESP's process: 13/14746-4 - Carnosine metabolism in skeletal muscle: a multi-approach study
• Grantee: Bruno Gualano
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/13552-2 - Reducing sedentary time in clinical populations: the take a stand for health study
• Grantee: Bruno Gualano
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
• Grantee: Ohara Augusto
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC