Photobiomodulation effect on angiogenic proteins produced and released by dental pulp cells

Author(s):Show less -

Ribeiro Vitor, Luciana Lourenco ^[1] ; Oliveira Prado Bergamo, Mariel Tavares ^[2] ; Lourenco-Neto, Natalino ^[2] ; Sakai, Vivien Thiemy ^[3] ; Oliveira, Rodrigo Cardoso ^[4] ; Cruvinel, Thiago ^[2] ; Rios, Daniela ^[2] ; Garlet, Gustavo Pompermaier ^[4] ; Santos, Carlos Ferreira ^[4] ; Andrade Moreira Machado, Maria Aparecida ^[2] ; Oliveira, Thais Marchini ^[2]

Affiliation

^[1] Univ Sagrado Coracao, Dept Pediat Dent, Rua Irma Arminda 10-15, BR-17011160 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP - Brazil

^[3] Univ Fed Alfenas, Sch Dent, Dept Clin & Surg, Alfenas, MG - Brazil

^[4] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 24, n. 12 APR 2020.
• Web of Science Citations: 0

Abstract

Objective To verify the photobiomodulation effect on angiogenic proteins produced and released by dental human pulpal fibroblasts (HPFs). Material and methods HPFs were irradiated with 660-nm low-level laser at fluences of 2.5 J/cm(2) and 3.7 J/cm(2). The control group was not irradiated. MTT, crystal violet, and ELISA assays respectively verified viability, proliferation, and angiogenic protein (supernatant/lysate) at 6 h, 12 h, and 24 h after photobiomodulation. Capillary-like structure formation assay verified functional role. Two-way ANOVA/Tukey's test and ANOVA/Bonferroni's multiple comparisons test respectively verified cell viability/proliferation and intragroup and intergroup comparisons of protein synthesis (p < 0.05). Results Irradiated and non-irradiated HPFs showed statistically similar cell viability and proliferation pattern. Intragroup comparisons showed similar patterns of protein synthesis for all groups: VEGF-A, VEGF-C, and vascular endothelial growth factor receptor 1 (VEGFR1) increased significantly in the supernatant, while FGF-2 and VEGF-A increased significantly in the lysate. The lower fluence significantly increased BMP-9 (6 h) in the supernatant and VEGFR1 (6 h and 12 h) and VEGF-D (24 h) in the lysate, while the higher fluence significantly increased BMP-9 (6 h) in the supernatant and VEGFR1 (12 h) in the lysate. Regardless of the time, both fluences statistically downregulated placental growth factor (PLGF) and PDGF secretion. Both fluences statistically decreased VEGF-A secretion (24 h) and PLGF production (6 h). Conclusion Photobiomodulation produced stimulatory effects on angiogenic protein secretion by pulp fibroblasts. In terms of photobiomodulation, over time, both fluences significantly increased the secretion of VEGF-A, VEGF-C, and VEGFR1 and significantly upregulated BMP-9 (6 h) in the supernatant; for capillary-like structure formation, the fluence of 2.5 J/cm(2) was better than the fluence of 3.7 J/cm(2). (AU)

• FAPESP's process: 18/20316-6 - Gene expression and protein synthesis analysis of dental pulp cells from deciduous teeth after the use of Low Intensity Laser.
• Grantee: Thais Marchini de Oliveira Valarelli
• Support Opportunities: Regular Research Grants