Fusarium oxysporum f. sp. phaseoli genetic variability assessed by new developed microsatellites

Author(s)

Graziéle R. Sasseron ^[1] ; Luciana L. Benchimol-Reis ^[2] ; Juliana M.K.C. Perseguini ^[3] ; Jean Fausto C. Paulino ^[4] ; Miklos M. Bajay ^[5] ; Sérgio A.M. Carbonell ^[6] ; Alisson F. Chiorato ^[7]

Affiliation

^[1] Instituto Agrônomico. Centro de Pesquisa em Recursos Genéticos - Brasil

^[2] Instituto Agrônomico. Centro de Pesquisa em Recursos Genéticos - Brasil

^[3] Universidade Tecnológica Federal do Paraná - Brasil

^[4] Instituto Agrônomico. Centro de Pesquisa em Recursos Genéticos - Brasil

^[5] Universidade do Estado de Santa Catarina. Centro de Educação Superior da Região Sul da (CERES). Departamento de Engenharia de Pesca e Ciências Biológicas (DEPB) - Brasil

^[6] Instituto Agrônomico. Centro de Análise e Pesquisa Tecnológica do Agronegócio dos Grãos e Fibras - Brasil

^[7] Instituto Agrônomico. Centro de Análise e Pesquisa Tecnológica do Agronegócio dos Grãos e Fibras - Brasil

• Document type: Journal article
• Source: GENETICS AND MOLECULAR BIOLOGY; v. 43, n. 2 2020-05-29.

Abstract

Abstract Fusarium oxysporum f. sp. phaseoli (Fop) J.B. Kendrich & W.C. Snyder is the causal agent of Fusarium wilt of common bean (Phaseolus vulgaris L.). The objective of this study was to develop microsatellite markers (SSRs) to characterize the genetic diversity of Fop. Two libraries enriched with SSRs were developed and a total of 40 pairs of SSRs were characterized. Out of these, 15 SSRs were polymorphic for 42 Fop isolates. The number of alleles varied from two to ten, with an average of four alleles per locus and an average PIC (Polymorphic Information Content) of 0.38. The genetic diversity assessed by microsatellites for Fop was low, as expected for an asexual fungus, and not associated with geographic origin, but they were able to detect enough genetic variability among isolates in order to differentiate them. Microsatellites are a robust tool widely used for genetic fingerprinting and population structure analyses. SSRs for Fop may be an efficient tool for a better understanding of the ecology, epidemiology and evolution of this pathogen. (AU)

• FAPESP's process: 11/05786-7 - Identification and characterization of physiological races of Fusarium oxysporum F. sp. phaseoli in common beans
• Grantee: Alisson Fernando Chiorato
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/12416-1 - Genetic diversity and plant-pathogen interaction for different physiologycal races of Fusarium oxysporium f. sp. phaseoli in common bean
• Grantee: Graziele Ramos Sasseron
• Support Opportunities: Scholarships in Brazil - Doctorate