Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS2 and Bioactivity Assessment

Author(s)

Bezerra, Taliana ^[1] ; Estevez, Mario ^[2] ; Lacerda, Jose Thalles ^[3] ; Dias, Meriellen ^[4] ; Juliano, Maria ^[3] ; Mendes, Maria Anita ^[4] ; Morgano, Marcelo ^[5] ; Pacheco, Maria Teresa ^[5] ; Madruga, Marta ^[1]

Affiliation

^[1] Univ Fed Paraiba, Technol Ctr, Dept Food Engn, BR-58051900 Joao Pessoa, Paraiba - Brazil

^[2] Univ Extremadura, IPROCAR Res Inst, Anim Prod & Food Sci Dept, Avda Ciencias S-N, Caceres 10003 - Spain

^[3] Univ Fed Sao Paulo, Dept Biophys, Rua Tres Maio 2, BR-100 Sao Paulo - Brazil

^[4] Sch Univ Sao Paulo, Dept Polytech, Dempster MS Lab Chem Engn, Sao Paulo Rua Lago 250, Bloco B 3, BR-05508080 Sao Paulo - Brazil

^[5] Inst Food Technol ITAL, Brasil Ave 2880, POB 139, BR-13070178 Campinas - Brazil

• Document type: Journal article
• Source: Molecules; v. 25, n. 7 APR 2020.
• Web of Science Citations: 0

Abstract

The production of bioactive peptides from organic by-waste materials is in line with current trends devoted to guaranteeing environmental protection and a circular economy. The objectives of this study were i) to optimize the conditions for obtaining bioactive hydrolysates from chicken combs and wattles using Alcalase, ii) to identify the resulting peptides using LC-ESI-MS2 and iii) to evaluate their chelating and antioxidant activities. The hydrolysate obtained using a ratio of enzyme to substrate of 5% (w/w) and 240 min of hydrolysis showed excellent Fe2+ chelating and antioxidant capacities, reducing Fe3+ and inhibiting 2, 2 `-Azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The mapping of ion distribution showed that a high degree of hydrolysis led to the production of peptides with m/z <= 400, suggesting low mass peptides or peptides with multiple charge precursor ions. The peptides derived from the proteins of cartilage like Collagen alpha-2(I), Collagen alpha-1(I), Collagen alpha-1(III) and elastin contributed to generation of bioactive compounds. Hydrolysates from chicken waste materials could be regarded as candidates to be used as ingredients to design processed foods with functional properties. (AU)

• FAPESP's process: 18/13588-0 - Multi - user equipment approved in grant 2012/50191-4: mass spectrometer impact II - Bruker
• Grantee: Maria Aparecida Juliano
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 12/50191-4 - Synthesis, kinetic studies and applications of substrates and inhibitors for proteolytic enzymes
• Grantee: Maria Aparecida Juliano
• Support Opportunities: Research Projects - Thematic Grants