Evaluation of biological activity of peptides released from the hydrolysis of caprine casein by proteases synthesized by filamentous fungi

• Grant number: 14/13700-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2014
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Roberto da Silva
• Grantee: José Erick Galindo Gomes
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated scholarship(s): 16/10289-6 - Purification and identification of peptides with biological activities, released from the hydrolysis of casein goats for protease synthesized by filamentous fungi, BE.EP.DR

Abstract

The search for natural compounds in the presence of biological activities that assist the physiological functions in organisms have driven research by the pharmaceutical industry and especially food. In this sense, bioactive peptides have a high potential for use in the food industry, especially for the development of functional foods and beverages. Consequently, they have become the stimulus by the industry and academia to develop techniques and processes with different sources for the proteolytic release of these substances. Hydrolysis of casein by specific microbial proteases such as filamentous fungi results in the release of various peptides with different biological activities. Thus, screening and evaluation of these peptides for their application, may represent an important contribution to the functional food sector. Therefore, the aim of this study is to evaluate the antioxidant, antimicrobial and antihypertensive activity of peptides released by hydrolysis of caprine casein using proteases produced by filamentous fungus previously selected. For this purpose, will be initially evaluated the potential hydrolysis of five filamentous fungi producers of proteases selected from a database of 70 species, according to the evaluation of the biological activities of their hydrolysates. Subsequently, the fungus rated "GRAS" (Generally Regarded the safe) to present in their hydrolyzed the best results for the antihypertensive activity, will be selected for the optimization of protease production, purification and partial enzymatic hydrolysis of casein. The peptides released shall be purified, identified, evaluated for biological activities proposed and characterized molecularly. Thus, separation and purification techniques such as electrophoresis and high performance liquid chromatography using iodines arrangement coupled to a mass spectrometer detector of the type LC-MS/MS will be employed for both reports in the literature of these biomolecules, and with the aim of enhance the achievement of bioactive peptides. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GALINDO GOMES, JOSE ERICK; EVARISTO DA SILVA NASCIMENTO, TALITA CAMILA; DE SOUZA-MOTTA, CRISTINA MARIA; AGAMEZ MONTALVO, GUALBERTO SEGUNDO; BOSCOLO, MAURICIO; GOMES, ELENI; MOREIRA, KEILA APARECIDA; PINTADO, MARIA MANUELA; DA SILVA, ROBERTO. Screening and application of fungal proteases for goat casein hydrolysis towards the development of bioactive hydrolysates. OURNAL OF FOOD MEASUREMENT AND CHARACTERIZATIO, v. 16, n. 6, p. 15-pg., 2022-08-12. (17/16482-5, 14/13700-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GOMES, José Erick Galindo. Evaluation of the biological activities of peptides released from the hydrolysis of caprine casein by proteases synthesized by filamentous fungi. 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.