Lead toxicity on a sentinel species subpopulation inhabiting mangroves with different status conservation

Author(s)

de Almeida Duarte, Luis Felipe ^[1] ; Blasco, Julian ^[2] ; Miranda Catharino, Marilia Gabriela ^[3] ; Moreira, Edson Goncalves ^[3] ; Trombini, Chiara ^[2] ; Nobre, Caio Rodrigues ^[1] ; Moreno, Beatriz Barbosa ^[1] ; de Souza Abessa, Denis Moledo ^[4] ; Seabra Pereira, Camilo Dias ^{[1, 5]}

Affiliation

^[1] Univ Fed Sao Paulo, Dept Ciencias Mar, Campus Baixada Santista, Rua Maria Maximo 168, BR-11030100 Santos, SP - Brazil

^[2] Inst Ciencias Marinas Andalucia ICMAN, Campus Rio San Pedro S-N, Cadiz - Spain

^[3] CNEN SP, IPEN, Av Prof Lineu Prestes 2242, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

^[4] Univ Estadual Sao Paulo UNESP, Ctr Invest Aquat Pollut & Ecotoxicol, Praca Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP - Brazil

^[5] Univ Santa Cecilia, Lab Ecotoxicol, Rua Oswaldo Cruz 266, BR-11045907 Santos, SP - Brazil

• Document type: Journal article
• Source: Chemosphere; v. 251, JUL 2020.
• Web of Science Citations: 1

Abstract

Lead is a priority pollutant introduced in the aquatic environment by different sources commonly located in estuarine regions, such as ports, marinas and industries. Environmental agencies around the world set the maximum allowable concentration of lead in effluents, surface water and sediment, but few studies reported its accumulation and chronic toxicity in mangrove benthic invertebrates using concentrations believed to be safe. In the case of Brazilian mangrove environments, Ucides cordatus is a crab species of choice to be used in bioaccumulation studies. We have assessed biomarkers' responses (DNA strand breaks, micronucleated cells, metallothioneins, enzymatic activity of aminolevulinic acid dehydratase and neutral red retention time) and the total bioaccumulation in six tissues of U. cordatus crabs resident to mangrove areas under different conservation status during a 28-day period bioassay. We also investigated Pb subcellular partition and biomarkers' responses using a supposedly safe concentration (10 mu g L-1). During the Pb exposure, the highest concentration of Pb was observed in crab gills. Crabs also showed a high ability to allocate Pb in detoxified forms. Multivariate analysis pointed out that bioaccumulation (total, active and detoxified) is linked to biomarkers. Even in supposedly safe dosage, U. cordatus triggered its defense mechanisms expressing more metallothioneins and presented relevant cyto-genotoxic damage. Our data suggest the development of biological tolerance to Pb in crabs from polluted areas. Our results provided a new insight about lead toxicity even at concentrations considered environmentally safe, which could support new strategies to manage estuarine areas considering their respective conservation status. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/13448-2 - Biological tolerance, sublethal effects and bioaccumulation in Ucides cordatus (Linnaeus, 1763) exposed to contaminants, via food and water
• Grantee: Luis Felipe de Almeida Duarte
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/05414-6 - Subcellular partitioning and specific biomarkers to trace metals exposure in the mangrove sentinel Ucides cordatus (Linnaeus, 1763).
• Grantee: Luis Felipe de Almeida Duarte
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 10/01552-9 - Genotoxic impact on populations of the crab, Ucides cordatus (Linnaeus, 1763) (Crustacea, Brachyura, Ucididae), in five mangrove areas at São Paulo State, Brazil.
• Grantee: Luis Felipe de Almeida Duarte
• Support Opportunities: Scholarships in Brazil - Doctorate