| Full text | |
| Author(s): |
Guedes, Wesley Nascimento
[1]
;
Lucena, Guilherme Nunes
[1, 2]
;
de Paula, Ariela Veloso
[3]
;
Costa Marques, Rodrigo Fernando
[2, 4]
;
Verbi Pereira, Fabiola Manhas
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Estadual Paulista Unesp, Inst Quim, Inst Pesquisa Bioenergia IPBEN, BR-14800060 Araraquara, SP - Brazil
[2] Univ Estadual Paulista Unesp, Inst Quim, Grp Mat Magnet & Coloides, BR-14800060 Araraquara, SP - Brazil
[3] Univ Estadual Paulista Unesp, Fac Ciencias Farmaceut, Rodovia Araraquara Jau, Km 1, BR-14800903 Araraquara, SP - Brazil
[4] Univ Estadual Paulista Unesp, Ctr Monitoramento & Pesquisa Qualidade Combustive, Biocombustiveis Petr & Derivados Cempeqc, Inst Quim, BR-14800060 Araraquara, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY; v. 7, n. 26, p. 27-35, JAN-MAR 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
This study describes the estimation of the enzymatic activity of endoglucanase based on the 3,5-dinitrosalicylic acid (DNS) method and carboxymethylcellulose (CMC) substrate using glucose as the analyte. As an alternative to measuring the colored product obtained by the reaction between glucose and DNS, a free software app for mobile devices was used for estimating the color intensity of solutions, replacing the need of a UV-Vis spectrophotometer (reference method). The app is able to convert images in color histograms to red (R), green (G) and blue (B) scales and to provide univariate and multivariate calculations of the image data. The chemometric technique partial least squares (PLS) was employed with the app and presented accurate results that were comparable to those of the reference method. A correlation coefficient of 0.983 was obtained for the linear range between 0.60 and 2.60 mg mL(-1) of glucose, and the standard error of cross-validation (SECV) was 0.219 mg mL(-1). The proposed method (imaging - PLS) was applied for samples of cellulase enzymes from Aspergillus niger and Trichoderma reesei, revealing an interesting alternative to estimate the enzymatic activity of endoglucanase. (AU) | |
| FAPESP's process: | 18/18212-8 - Development of chemometric models for determination of solid impurities in raw sugarcane using energy dispersive X-ray fluorescence |
| Grantee: | Fabiola Manhas Verbi Pereira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/01102-8 - Multi-user equipament approved in grant 2018/18212-8: X-Ray Fluorescence spectrometer |
| Grantee: | Fabiola Manhas Verbi Pereira |
| Support Opportunities: | Multi-user Equipment Program |