Studying the link between physiological performance of Crotalaria ochroleuca and the distribution of Ca, P, K and S in seeds with Xray fluorescence

Author(s):Show less -

Cotrim, Mayara Favero ^[1] ; da Silva, Josue Bispo ^[2] ; dos Santos Lourenco, Flavia Mendes ^[3] ; Teixeira, Anielli Verzotto ^[1] ; Gava, Ricardo ^[1] ; Alves, Charline Zaratin ^[1] ; da Silva Candido, Ana Carina ^[1] ; Silva Campos, Cid Naudi ^[1] ; Pereira, Marcio Dias ^[4] ; Torres, Salvador Barros ^[5] ; Bacchetta, Gianluigi ^[6] ; Teodoro, Paulo Eduardo ^[1]

Affiliation

^[1] Fed Univ Mato Grosso do Sul UFMS, Chapadao Do Sul, MS - Brazil

^[2] Fed Univ Mato Grosso do Sul UFMS, Tres Lagoas, MS - Brazil

^[3] State Univ Sao Paulo UNESP, Ilha Solteira, SP - Brazil

^[4] Fed Univ Rio Grande do Norte UFRN, Natal, RN - Brazil

^[5] Fed Univ Semiarid UFERSA, Mossoro, RN - Brazil

^[6] Univ Cagliari, Cagliari, CA - Italy

• Document type: Journal article
• Source: PLoS One; v. 14, n. 9 SEP 26 2019.
• Web of Science Citations: 0

Abstract

This study describes the use of X-ray fluorescence spectroscopy in Crotalaria ochroleuca seed technology. This work evaluated X-ray fluorescence techniques to estimate the physiological performance of different C. ochroleuca seed coat colours based on the concentration and distribution of Ca, P, K, and S in seed structures. The treatments consisted of seeds separated by coat colours (yellow, green, and red) and a control treatment (colour mix according to their natural occurrence in commercial lots), and was carried out in a completely randomized design, with four replications. The physiological performance was evaluated by analyzing the water content, germination, first germination count, germination speed index, electrical conductivity, seedling emergence, and seedling length and dry mass. X-ray fluorescence spectroscopy techniques were carried out with quantitative analyses (Ca, P, K, and S concentration in the seed coat and the whole seed) and qualitative analyses (macronutrient mapping). The EDXRF and mu-XRF techniques are efficient and promising to differentiate the physiological performance of C. ochroleuca seeds, based on the concentration and distribution of Ca, P, K, and S in different structures. Ca is predominant in the seed coat, and K, S, and P are found throughout the embryonic axis. Seeds of yellow and green coats have higher nutrients concentration and distribution in the embryonic axis, revealing high germinative capacity and physiological performance. Seeds of red coat have higher nutrients concentration in the seed coat and lower assimilation, showing less vigour, which interferes directly in the quality of commercial lots. (AU)

• FAPESP's process: 15/19121-8 - Multi-User Equipment approved in grant 2015/05942-0: micro X-Ray Fluorescence system
• Grantee: Hudson Wallace Pereira de Carvalho
• Support Opportunities: Multi-user Equipment Program