Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model

Author(s):Show less -

Oliveira, Fernando A. ^[1] ; Nucci, Mariana P. ^[2] ; Filgueiras, Igor S. ^[1] ; Ferreira, Joao M. ^[1] ; Nucci, Leopoldo P. ^[3] ; Mamani, Javier B. ^[1] ; Alvieri, Fernando ^[1] ; Souza, Lucas E. B. ^[4] ; Rego, Gabriel N. A. ^[1] ; Kondo, Andrea T. ^[1] ; Hamerschlak, Nelson ^[1] ; Gamarra, Lionel F. ^[1]

Affiliation

^[1] Hosp Israelita Albert Einstein, BR-05652900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, LIM44 Hosp Clin HCFMUSP, Fac Med, BR-01246903 Sao Paulo - Brazil

^[3] Ctr Univ Planalto Cent, BR-72445020 Brasilia, DF - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Review article
• Source: CELLS; v. 9, n. 4 APR 2020.
• Web of Science Citations: 0

Abstract

The hematopoietic stem cell engraftment depends on adequate cell numbers, their homing, and the subsequent short and long-term engraftment of these cells in the niche. We performed a systematic review of the methods employed to track hematopoietic reconstitution using molecular imaging. We searched articles indexed, published prior to January 2020, in PubMed, Cochrane, and Scopus with the following keyword sequences: (Hematopoietic Stem Cell OR Hematopoietic Progenitor Cell) AND (Tracking OR Homing) AND (Transplantation). Of 2191 articles identified, only 21 articles were included in this review, after screening and eligibility assessment. The cell source was in the majority of bone marrow from mice (43%), followed by the umbilical cord from humans (33%). The labeling agent had the follow distribution between the selected studies: 14% nanoparticle, 29% radioisotope, 19% fluorophore, 19% luciferase, and 19% animal transgenic. The type of graft used in the studies was 57% allogeneic, 38% xenogeneic, and 5% autologous, being the HSC receptor: 57% mice, 9% rat, 19% fish, 5% for dog, porcine and salamander. The imaging technique used in the HSC tracking had the following distribution between studies: Positron emission tomography/single-photon emission computed tomography 29%, bioluminescence 33%, fluorescence 19%, magnetic resonance imaging 14%, and near-infrared fluorescence imaging 5%. The efficiency of the graft was evaluated in 61% of the selected studies, and before one month of implantation, the cell renewal was very low (less than 20%), but after three months, the efficiency was more than 50%, mainly in the allogeneic graft. In conclusion, our review showed an increase in using noninvasive imaging techniques in HSC tracking using the bone marrow transplant model. However, successful transplantation depends on the formation of engraftment, and the functionality of cells after the graft, aspects that are poorly explored and that have high relevance for clinical analysis. (AU)

• FAPESP's process: 16/21470-3 - Therapeutic action of mesenchymal stem cells from human bone marrow labeled with multimodal nanoparticles in diabetic rats subjected to stroke: study of cellular, molecular and functional mechanisms.
• Grantee: Lionel Fernel Gamarra Contreras
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/50983-3 - INCT 2014: complex fluids
• Grantee: Antonio Martins Figueiredo Neto
• Support Opportunities: Research Projects - Thematic Grants