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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantification of lactose and lactulose in hydrolysed-lactose UHT milk using capillary zone electrophoresis

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Author(s):
de Oliveira Neves, Leandra N. [1] ; Leal de Oliveira, Marcone A. [1]
Total Authors: 2
Affiliation:
[1] Univ Fed Juiz de Fora, Dept Chem, BR-36036330 Juiz De Fora, MG - Brazil
Total Affiliations: 1
Document type: Journal article
Source: INTERNATIONAL DAIRY JOURNAL; v. 106, JUL 2020.
Web of Science Citations: 3
Abstract

A new method for quantification of lactose and lactulose in hydrolysed-lactose UHT milk, using capillary zone electrophoresis with indirect detection in the ultraviolet region (CZE-UV), is proposed. A 3(3) Box-Behnken design for instrumental electrophoretic parameters optimisation was used, with the best conditions shown to be an injection pressure of 15 mbar 2 s, applied voltage of -20 kV, and cartridge temperature of 20 degrees C. Extraction procedures were investigated and protein precipitation using trichloroacetic acid solution was shown to give the best results. Limits of quantification for lactose and lactulose were 0.024 g 100 mL(-1) and 0.0094 g 100 mL(-1), respectively. Recovery levels ranging from 86% to 102% were obtained. The method was successful applied to several hydrolysed-lactose and regular UHT milk samples from six commercial leading brands. Results showed that all hydrolysed-lactose samples are in accordance with the legal recommendations to be classified as ``lactose-free{''} products. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Marco Aurelio Zezzi Arruda
Support Opportunities: Research Projects - Thematic Grants