| Full text | |
| Author(s): |
Santos, T. S.
[1]
;
Campos, F. B.
[1]
;
Padovani, N. F. A.
[1]
;
Dias, M.
[2]
;
Mendes, M. A.
[2]
;
Maffei, D. F.
[1, 3]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Ave Padua Dias 11, CP9, BR-13418900 Piracicaba, SP - Brazil
[2] Univ Sao Paulo, Polytech Sch, Dept Chem Engn, Dempster MS Lab, Sao Paulo, SP - Brazil
[3] CEPID, Food Res Ctr FoRC, Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Letters in Applied Microbiology; v. 71, n. 2 MAY 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
The present study shows the results of the microbiological quality and safety of minimally processed vegetables sold in supermarkets and grocery stores located in the city of Piracicaba, SP, Brazil. A total of 100 samples were collected and submitted to enumeration of total coliforms, coliforms at 45 degrees C and generic Escherichia coli using the standard most probable number (MPN) method, in addition to enumeration of total Enterobacteriaceae by plating on MacConkey agar. Moreover, colonies of Enterobacteriaceae were randomly selected and submitted to identification on a MALDI-TOF MS Biotyper (TM). Samples were also tested for Salmonella spp. according to the ISO 6579:2002 method. The mean count of total coliforms was 2 center dot 9 +/- 0 center dot 5 log MPN per g. For coliforms at 45 degrees C, 20 samples were positive (mean 1 center dot 5 +/- 1 center dot 0 log MPN per g). Generic E. coli was detected in 16 samples (mean 1 center dot 4 +/- 0 center dot 9 log MPN per g) and only one was positive for Salmonella. The mean count of total Enterobacteriaceae was 6 center dot 5 +/- 1 center dot 2 log CFU per g and the most frequent genera identified by MALDI-TOF were Enterobacter (25 center dot 9%), Pantoea (9 center dot 6%) and Rahnella (9 center dot 0%). Overall, results point to poor microbiological quality of a few samples, indicating hygiene failure during their processing. This can pose health risks to consumers, mainly because these products were labelled as sanitized and marketed as ready-to-eat. (AU) | |
| FAPESP's process: | 13/07914-8 - FoRC - Food Research Center |
| Grantee: | Bernadette Dora Gombossy de Melo Franco |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |