Herpesviruses oral shedding and viremia in renal transplant recipients: A longitudinal study

Author(s)

de Santana Sarmento, Dmitry Jose ^[1] ; Tozetto-Mendoza, Tania Regina ^[2] ; Fernandes de Souza, Ana Carolina Mamana ^[2] ; Maciel, Rafael ^[3] ; Paiao, Heuder ^[2] ; Lima, Silvia Helena ^[2] ; Cristelli, Marina ^[4] ; Medina de Abreu Pestana, Jose Osmar ^[4] ; Braz-Silva, Paulo Henrique ^{[1, 2]} ; Gallottini, Marina ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Inst Trop Med Sao Paulo, Lab Virol, Sao Paulo - Brazil

^[3] Social Inst Healthcare, Div Renal Transplantat, Campina Grande, Paraiba - Brazil

^[4] Univ Fed Sao Paulo, Kidney & Hypertens Hosp, Div Renal Transplantat, Sao Paulo - Brazil

• Document type: Journal article
• Source: TRANSPLANT INFECTIOUS DISEASE; v. 22, n. 4 JUN 2020.
• Web of Science Citations: 0

Abstract

Background The objective was to assess the oral shedding and viremia of human herpesviruses in renal transplant recipients. Methods This is a cohort study in which the participants were examined in three different periods: the first within 24 hours before renal transplantation and the second and third ones 15-20 and 45-60 days after the transplantation. Mouthwash and blood samples were collected in each period and then submitted to screening for the presence of eight types of human herpesviruses by using multiplex PCR. Results HSV-1 and EBV were more frequent in the saliva after renal transplantation, 15- to 20-day period after the transplant. EBV was found in the saliva of 26 (35.6%) patients before renal transplantation and in 56.2% and 46.6% of them, in the 15- to 20-day and 45- to 60-day periods after the transplant, respectively. High detection rates (75.3%-78.1%) were found for HHV-7 despite the lack of significant variations between the study periods. There was no concordance between herpesviruses oral shedding and viremia. Conclusion We concluded that the pattern of excretion of HSV-1 and EBV in saliva is changed immediately after renal transplantation, increasing in the 15- to 20-day period after the transplant surgery. No concordance between herpesviruses oral shedding and viremia was observed. (AU)

• FAPESP's process: 17/07778-8 - Impact of renal transplantation on oral health and dental management
• Grantee: Marina Helena Cury Gallottini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/07727-9 - Evaluation of immunoregulatory microenvironment of oral premalignant lesions, squamous cell carcinomas and oropharyngeal carcinomas related to HPV or not.
• Grantee: Paulo Henrique Braz da Silva
• Support Opportunities: Regular Research Grants