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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DNA mini-barcoding of leporids using noninvasive fecal DNA samples and its significance for monitoring an invasive species

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Author(s):
Rodrigues, Nayra T. [1] ; Saranholi, Bruno H. [1] ; Angeloni, Thais A. [1] ; Pasqualotto, Nielson [2, 3, 4] ; Chiarello, Adriano G. [3] ; Galetti, Jr., Pedro M. [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Ctr Energia Nucl Agr, Piracicaba - Brazil
[3] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Programa Interunidades Posgrad Ecol Aplicada PPGI, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil
Total Affiliations: 4
Document type: Journal article
Source: ECOLOGY AND EVOLUTION; v. 10, n. 12 JUN 2020.
Web of Science Citations: 0
Abstract

Introduced in South America at the end of the 19th century, the European hare population has expanded dramatically and now represents a risk to native Brazilian forest rabbits. Monitoring the invasive Lepus europaeus and its coexistence with native Sylvilagus brasiliensis is a challenge that can be efficiently addressed by the use of molecular tools. This work describes a set of primers useful for amplifying three mini-barcodes for the molecular identification of both invasive and native leporid species using degraded fecal DNA. In addition, tests in silico indicate that these mini-barcodes can successfully amplify the DNA sequences of a number of leporids. These mini-barcodes constitute a powerful tool for the monitoring and management of the invasive L. europaeus and the conservation of native rabbits. (AU)

FAPESP's process: 16/19106-1 - Mammal occurrence and biological invasion in Cerrado remnants of agricultural landscapes
Grantee:Adriano Garcia Chiarello
Support Opportunities: Regular Research Grants
FAPESP's process: 13/24453-4 - Genetic diversity of felids (Puma concolor, Leopardus pardalis, Leopardus tigrinus and Puma yagouaroundi) and the mesopredator (Leopardus pardalis) presence effect on density and genetic diversity of the smaller felid species.
Grantee:Bruno Henrique Saranholi
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 18/11788-1 - European Hare invasion and its association with structure, composition and agricultural crops cycle in the interior of São Paulo
Grantee:Nielson Aparecido Pasqualotto Salvador
Support Opportunities: Scholarships in Brazil - Doctorate