| Full text | |
| Author(s): |
Rossini, Eduardo Luiz
[1, 2]
;
Kulyk, Dmytro S.
[1]
;
Ansu-Gyeabourh, Emelia
[1]
;
Sahraeian, Taghi
[1]
;
Pezza, Helena Redigolo
[2]
;
Badu-Tawiah, Abraham K.
[1]
Total Authors: 6
|
| Affiliation: | [1] Ohio State Univ, Dept Chem & Biochem, Columbus, OH 43210 - USA
[2] Sao Paulo State Univ UNESP, Inst Chem, Dept Analyt Chem, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY; v. 31, n. 6, p. 1212-1222, JUN 3 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
In this study, the direct analysis of doping agents in urine samples with no sample preparation by a modified paper spray mass spectrometry (PS-MS) methodology has been demonstrated for the first time. We have described a paper surface treatment with trichloromethylsilane using a gas-phase reaction to increase the ionization of target compounds. This approach was applied for the analysis of two classes of banned substances in urine samples: anabolic agents (trenbolone and clenbuterol) and diuretics (furosemide and hydrochlorothiazide). Under optimized conditions, the developed methods presented satisfactory repeatability, and an analysis of variance showed linearity without lack-of-fit. Highly sensitive detections as low as sub-nanogram per milliliter levels, which is below the minimum required performance levels proposed by the World Anti-Doping Agency, have been reached using the hydrophobic PS-MS analysis without any preconcentration and cleanup step. (AU) | |
| FAPESP's process: | 18/05993-1 - Paper-spray mass spectrometry as a tool for doping detection |
| Grantee: | Eduardo Luiz Rossini |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |