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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photodynamic inactivation of Candida albicans and Candida tropicalis with aluminum phthalocyanine chloride nanoemulsion

Full text
Author(s):
Rodrigues, Gabriela Braga [1] ; Pinto, Marcelo Rodrigues [2] ; Leite Braga, Gilberto Ubida [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: FUNGAL BIOLOGY; v. 124, n. 5, p. 297-303, MAY 2020.
Web of Science Citations: 1
Abstract

The in vitro susceptibilities of Candida albicans and Candida tropicalis to Antimicrobial Photodynamic Treatment with aluminum phthalocyanine chloride in nanoemulsion (ClAlPc/NE) were investigated. PS concentration- and fluence-dependent cell survival after APDT were compared before and after unbound extracellular PS had been washed out. The PS uptake and its subcellular localization were also determined. Exposure to light in the absence of the PS and treatment with the PS in the absence of light did not kill the fungi. APDT with ClAlPc/NE resulted in a reduction of five orders of magnitude in viability for C. albicans and between four and five orders of magnitude for C. tropicalis. Washing the cells to remove unbound PS before light exposure did not impair fungal inactivation, suggesting that cell photosensitization was mainly carried out by cell bound ClAlPc. The degree of ClAlPc uptake was dependent on its concentration. Internalization of ClAlPc by C. albicans and C. tropicalis was confirmed by confocal fluorescence microscopy that showed the PS does not penetrate the nucleus and instead accumulates in specific regions of the cytoplasm. Our results show that incorporating the water-insoluble ClAlPc into a nanoemulsion leads to an efficient formulation capable of photoinactivating both Candida species. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/22933-6 - Mechanistic study of the photosensitization of Candida and Trichophyton species
Grantee:Gabriela Braga Rodrigues
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/15204-8 - Mechanistic study of the photodynamic inactivation of human and plant pathogenic fungi
Grantee:Gilberto Úbida Leite Braga
Support Opportunities: Regular Research Grants
FAPESP's process: 16/11386-5 - Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum
Grantee:Gilberto Úbida Leite Braga
Support Opportunities: Regular Research Grants
FAPESP's process: 18/20571-6 - International Symposium on Fungal Stress - ISFUS
Grantee:Drauzio Eduardo Naretto Rangel
Support Opportunities: Organization Grants - Scientific Meeting