Effects of ultraviolet light and curcumin-mediated photodynamic inactivation on microbiological food safety: A study in meat and fruit

Author(s)

Correa, Thaila Quatrini ^[1] ; Blanco, Kate Cristina ^{[1, 2]} ; Garcia, Erica Boer ^{[1, 2]} ; Lara Perez, Shirly Marleny ^{[1, 3, 2]} ; Chianfrone, Daniel Jose ^{[1, 2]} ; Morais, Vinicius Sigari ^{[1, 2]} ; Bagnato, Vanderlei Salvador ^{[4, 1, 2]}

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil

^[2] Bagnato, Vanderlei Salvador, Texas A\&M Univ, College Stn, TX USA.Correa, Thaila Quatrini, Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, PPG Biotec, BR-13565905 Sao Carlos, SP - Brazil

^[4] Texas A&M Univ, College Stn, TX - USA

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 30, JUN 2020.
• Web of Science Citations: 0

Abstract

Background: About one-third of the food produced in the world is lost or wasted every year. Contamination can cause significant food loss throughout the entire supply chain, including harvesting, processing, storage, and transport to consumers. This study evaluated ultraviolet-C (UV-C) light and curcumin-mediated photodynamic inactivation (PDI) for the decontamination of meat and fruit. Methods: The cut pieces of food samples contaminated with E. coli. or S. aureus were submitted to photonic treatments. For UV-C, samples were irradiated with UV-C lamps (254 nm) for 0, 1, 2, 3, 4, 5 and 10 min. For PDI, samples were incubated using 40 and 80 mu M curcumin and irradiated with 450 nm at 5, 10, and 15 J/cm(2) of light doses. The microbiological analysis was performed by counting the colony-forming unit (CFU). Results: UV-C irradiation reduced the number of E. coli in beef by (1.0 +/- 0.2) log(10) CFU/mL after 5 min of exposure. In chicken and pork, the numbers of E. coli were reduced by (1.6 +/- 0.7) log(10) CFU/mL and (1.6 +/- 0.4) log(10) CFU/mL after 4 and 10 min of irradiation, respectively. In apple the reductions were (3.2 +/- 0.4) and (3.8 +/- 0.2) log(10) CFU/mL after 5 and 10 min of UV-C irradiation, respectively. PDI (40 mu M, 15 J/cm(2)) reduced the number of S. aureus by (1.5 +/- 0.2), (1.4 +/- 0.2) and (0.6 +/- 0.4) log(10) CFU/mL in beef, chicken, and pork meat samples, respectively. In apple the greatest reduction was (2.0 +/- 0.4) log(10) CFU/mL using 80 mu m and 10 J/cm(2). Conclusion: UV-C irradiation and PDI had an antimicrobial effect in food and our findings indicated that the greatest effect was achieved in apples. Therefore, these techniques may be useful to reduce E. coli and S. aureus contamination levels on the surface of meats and fruits, being promising for applications in the field of microbiological food safety. (AU)

• FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
• Grantee: Igor Polikarpov
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants