Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bioactive Properties of Syzygium cumini (L.) Skeels Pulp and Seed Phenolic Extracts

Full text
Author(s):
Santos, Catarina A. [1] ; Almeida, Felipe A. [2] ; Quecan, Beatriz X. V. [1, 3] ; Pereira, Patricia A. P. [1, 4] ; Gandra, Kelly M. B. [1, 4] ; Cunha, Luciana R. [4] ; Pinto, Uelinton M. [1, 3]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Food Res Ctr, Fac Pharmaceut Sci, Sao Paulo - Brazil
[2] Univ Fed Juiz de Fora, Dept Nutr, Governador Valadares - Brazil
[3] Cunha, Luciana R., Univ Fed Ouro Preto, Dept Foods, Ouro Preto, Brazil.Santos, Catarina A., Univ Sao Paulo, Food Res Ctr, Fac Pharmaceut Sci, Sao Paulo - Brazil
[4] Univ Fed Ouro Preto, Dept Foods, Ouro Preto - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FRONTIERS IN MICROBIOLOGY; v. 11, MAY 27 2020.
Web of Science Citations: 0
Abstract

The emergence of bacterial strains resistant to different antibiotics has prompted the search for new sources of antimicrobial compounds. Studies have shown that jambolan {[}Syzygium cumini (L.) Skeels], a tropical fruit from the Mirtaceae family, contains a great variety of phytochemical compounds with high antioxidant and antimicrobial activity. This study aimed to determine the centesimal composition and physicochemical characteristics of the pulp and seed of S. cumini (L.) Skeels, as well as the content of total phenolic compounds and the antioxidant, antibacterial, antibiofilm and anti-quorum sensing (QS) activities of the phenolic extracts obtained from the pulp and the seeds of this fruit. The in vitro antibacterial and anti-QS activities of active films incorporating phenolic extracts were also evaluated. Additionally, we performed molecular docking of phenolic compounds present in jambolan with the CviR QS regulator of Chromobacterium violaceum. The composition and physicochemical characteristics of the samples presented similar values to those found for the species. However, the seed phenolic extract had a higher content of phenolic compounds and antioxidant activity than the pulp. Both phenolic extracts presented antibacterial activity against Aeromonas hydrophila, C. violaceum, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica serovar Typhimurium, Serratia marcescens, Listeria monocytogenes, and Staphylococcus aureus. The seed phenolic extract was particularly inhibitory against S. aureus. The pulp phenolic extract inhibited swarming motility and biofilm formation of A. hydrophila, E. coli, and S. marcescens in sub-MIC concentrations. The pulp and seed phenolic extracts inhibited violacein production in C. violaceum. Films incorporating both phenolic extracts inhibited the growth of bacteria, particularly Pseudomonas fluorescens, L. monocytogenes, and S. aureus, as well as QS in C. violaceum. Molecular docking showed that a variety of compounds found in pulp and seed extracts of jambolan, particularly chlorogenic acid and dihydroquercetin, potentially bind CviR protein and may interfere with QS. Our results indicate that pulp and seed of jambolan are good sources of antibacterial, antibiofilm, and anti-QS compounds that can be used in the development of natural preservatives and for application in antibacterial active films. (AU)

FAPESP's process: 13/07914-8 - FoRC - Food Research Center
Grantee:Bernadette Dora Gombossy de Melo Franco
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC