| Full text | |
| Author(s): |
Lazarini, Josy Goldoni
[1]
;
Franchin, Marcelo
[1, 2, 3]
;
Soares, Jackeline Cintra
[4]
;
Nani, Bruno Dias
[1, 2]
;
Massarioli, Adna Prado
[4]
;
de Alencar, Severino Matias
[4]
;
Rosalen, Pedro Luiz
[1, 2, 5]
Total Authors: 7
|
| Affiliation: | [1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[2] Rosalen, Pedro Luiz, Fed Univ Alfenas UNIFAL, Biol Sci Grad Program, Alfenas, MG, Brazil.Lazarini, Josy Goldoni, Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[3] Fed Univ Alfenas UNIFAL, Fac Dent, Alfenas, MG - Brazil
[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Piracicaba, SP - Brazil
[5] Fed Univ Alfenas UNIFAL, Biol Sci Grad Program, Alfenas, MG - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | PLoS One; v. 15, n. 6 JUN 9 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Brazilian native fruits are a rich source of polyphenolic compounds that can act as anti-inflammatory and antioxidant agents. Here, we determined the polyphenolic composition, anti-inflammatory mechanism of action, antioxidant activity and systemic toxicity in Galleria mellonella larvae of Eugenia selloi B.D.Jacks. (synonym Eugenia neonitida Sobral) extract (Ese) and its polyphenol-rich fraction (F3) obtained through bioassay-guided fractionation. Phenolic compounds present in Ese and F3 were identified by LC-ESI-QTOF-MS. The anti-inflammatory activity of Ese and F3 was tested in vitro and in vivo through NF-kappa B activation, cytokine release and neutrophil migration assays. The samples were tested for their effects against reactive species (ROO center dot, O2 center dot-, HOCl and NO center dot) and for their toxicity in Galleria mellonella larvae model. The presence of hydroxybenzoic acid, ellagitannins and flavonoids was identified. Ese and F3 reduced NF-kappa B activation, cytokine release and neutrophil migration, with F3 being three-fold more potent. Overall, F3 exhibited strong antioxidant effects against biologically relevant radicals, and neither Ese nor F3 were toxic to G. mellonella larvae. In conclusion, Ese and F3 revealed the presence of phenolic compounds that decreased the inflammatory parameters evaluated and inhibited reactive oxygen/nitrogen species. E. selloi is a novel source of bioactive compounds that may provide benefits for human health. (AU) | |
| FAPESP's process: | 16/02926-6 - Phytochemistry and bioprospection of antioxidant and anti-inflammatory activities from unexplored Brasilian native fruits. |
| Grantee: | Josy Goldoni Lazarini |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/09898-0 - Bioprospection of novel anti-inflammatory molecules from natural Brazilian native products |
| Grantee: | Pedro Luiz Rosalen |
| Support Opportunities: | Regular Research Grants |