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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic and biochemical evidence for redundant pathways leading to mycosporine-like amino acid biosynthesis in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024

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Author(s):
Geraldes, Vanessa [1] ; de Medeiros, Livia Soman [2] ; Lima, Stella T. [3] ; Alvarenga, Danillo Oliveira [3, 4] ; Gacesa, Ranko [5] ; Long, Paul F. [1, 5] ; Fiore, Marli Fatima [3] ; Pinto, Ernani [1, 3]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Pharmaceut Sci, BR-05508000 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Chem, BR-04021001 Diadema, SP - Brazil
[3] Univ Sao Paulo, Ctr Nucl Energy Agr, Piracicaba, SP - Brazil
[4] Univ Helsinki, Dept Microbiol, Helsinki 00100 - Finland
[5] Kings Coll London, Fac Life Sci & Med, Inst Pharmaceut Sci, London WC2R 2LS - England
Total Affiliations: 5
Document type: Journal article
Source: ALGAE; v. 35, n. 2, p. 177-187, JUN 2020.
Web of Science Citations: 2
Abstract

Cyanobacteria have been widely reported to produce a variety of UV-absorbing mycosporine-like amino acids (MAAs). Herein, we reported production of the unusual MAA, mycosporine-glycine-alanine (MGA) in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024 using a newly developed UHPLC-DAD-MS/HRMS (ultra-high performance liquid chromatography-diode array detection-high resolution tandem mass spectrometry) method. MGA had previously been first identified in a red-algae, but S. torques-reginae strain ITEP-024 is the first cyanobacteria to be reported as an MGA producer. Herein, the chemical structure of MGA is fully elucidated from one-dimensional / two-dimensional nuclear magnetic resonance and HRMS data analyses. MAAs are unusually produced constitutively in S. torques-reginae ITEP-024, and this production was further enhanced following UV-irradiance. It has been proposed that MAA biosynthesis proceeds in cyanobacteria from the pentose phosphate pathway intermediate sedoheptulose 7-phosphate. Annotation of a gene cluster encoded in the genome sequence of S. torques-reginae ITEP-024 supports these gene products could catalyse the biosynthesis of MAAs. However, addition of glyphosate to cultures of S. torques-reginae ITEP-024 abolished constitutive and ultra-violet radiation induced production of MGA, shinorine and porphyra-334. This finding supports involvement of the shikimic acid pathway in the biosynthesis of MAAs by this species. (AU)

FAPESP's process: 14/50420-9 - Secondary metabolites from aquatic microrganisms and their impact on seafood and freshwater fish aquaculture
Grantee:Ernani Pinto Junior
Support type: Regular Research Grants
FAPESP's process: 15/22742-4 - Identification of new bioactive secondary metabolites from microalgae by integrated analytical approaches
Grantee:Lívia Soman de Medeiros
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/01563-2 - Investigating the secondary metabolism of Brasilonema (Cyanobacteria: Scytonemataceae)
Grantee:Danillo Oliveira de Alvarenga
Support type: Scholarships abroad - Research Internship - Post-doctor