| Full text | |
| Author(s): |
Oliveira Monteiro, Lummy Maria
[1]
;
Sanches-Medeiros, Ananda
[1]
;
Westmann, Caua Antunes
[1]
;
Silva-Rocha, Rafael
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch FMRP, Ribeirao Preto - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 8, JUN 18 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Bacterial promoters are usually formed by multiplecis-regulatory elements recognized by a plethora of transcriptional factors (TFs). From those, global regulators are key elements since these TFs are responsible for the regulation of hundreds of genes in the bacterial genome. For instance, Fis and IHF are global regulators that play a major role in gene expression control inEscherichia coli, and usually, multiplecis-regulatory elements for these proteins are present at target promoters. Here, we investigated the relationship between the architecture of thecis-regulatory elements for Fis and IHF inE. coli. For this, we analyze 42 synthetic promoter variants harboring consensuscis-elements for Fis and IHF at different distances from the core -35/-10 region and in various numbers and combinations. We first demonstrated that although Fis preferentially recognizes its consensuscis-element, it can also recognize, to some extent, the consensus-binding site for IHF, and the same was true for IHF, which was also able to recognize Fis binding sites. However, changing the arrangement of thecis-elements (i.e., the position or number of sites) can completely abolish the non-specific binding of both TFs. More remarkably, we demonstrated that combiningcis-elements for both TFs could result in Fis and IHF repressed or activated promoters depending on the final architecture of the promoters in an unpredictable way. Taken together, the data presented here demonstrate how small changes in the architecture of bacterial promoters could result in drastic changes in the final regulatory logic of the system, with important implications for the understanding of natural complex promoters in bacteria and their engineering for novel applications. (AU) | |
| FAPESP's process: | 18/04810-0 - Deconstructing complexity in the regulatory network for biofilm formation in gram-negative bacteria |
| Grantee: | Ananda Sanches Medeiros |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters |
| Grantee: | Rafael Silva Rocha |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 16/05472-6 - Synthetic biology approaches to engineer/mine novel regulatory elements in bacteria |
| Grantee: | Cauã Antunes Westmann |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/19179-9 - Deciphering the architecture/function relationship in complex bacterial promoters through synthetic biology approaches |
| Grantee: | Lummy Maria Oliveira Monteiro |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/50116-6 - Loving the poison: molecular basis for metabolism and toxicity of the widely-used food preservative, propionate in Pseudomonas aeruginosa |
| Grantee: | Rafael Silva Rocha |
| Support Opportunities: | Regular Research Grants |