| Full text | |
| Author(s): |
Sartori, Luciana
[1]
;
Sellera, Fabio P.
[2]
;
Moura, Quezia
[3]
;
Cardoso, Brenda
[4]
;
Fontana, Herrison
[4]
;
Cortes, Lucas A.
[5]
;
Cerdeira, Louise
[4]
;
Lincopan, Nilton
[4, 1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharm, Dept Clin Anal, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo - Brazil
[3] Fed Univ Grande Dourados, Fac Hlth Sci, Dourados, MS - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[5] Special Care Vet Intensivist, Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 21, p. 186-187, JUN 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Objectives: Klebsiella pneumoniae has emerged as one of the major pathogens of humans and companion animals. Moreover, polymyxin resistance in K. pneumoniae is increasingly reported worldwide, mainly among extended-spectrum p-lactamase (ESBL)- and/or carbapenemase-producing isolates. The aim of this study was to report the draft genome sequence of a polymyxin-resistant, ESBL-producing K. pneumoniae isolate (14CSI) from a hospitalised domestic cat in Brazil. Methods: Whole-genome sequencing of strain 14CSI was performed on an Illumina NextSeq platform and the genome was de novo assembled using Velvet v.1.2.10. Data analysis was performed using bioinformatics tools available from the Center for Genomic Epidemiology and the Institut Pasteur database. Results: The genome size of strain 14CSI was calculated at 5 260 459 bp, with a GC content of 57.3% and comprising 5294 total genes, 28 tRNAs, 7 rRNAs, 8 ncRNAs and 237 pseudogenes. Klebsiella pneumoniae strain 14CSI belongs to sequence type 491 (ST491), presents a mutation (A14S) in the mgrB gene and coharbours bla(CTX-M-8) and qnrE1 genes. Genes conferring resistance to heavy metals were further identified. Conclusion: This draft genome could be used as a reference sequence for comparative analysis of polymyxin-resistant and/or CTX M 8 producing K. pneumoniae strains circulating at the human-animal interface. (C) 2020 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. (AU) | |
| FAPESP's process: | 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil |
| Grantee: | Nilton Erbet Lincopan Huenuman |
| Support Opportunities: | Regular Research Grants |