Biosynthesis of natural colorant by Talaromyces amestolkiae: Mycelium accumulation and colorant formation in incubator shaker and in bioreactor

Author(s)

de Oliveira, Fernanda ^[1] ; Ferreira, Laura Carmona ^[1] ; Baptista Neto, Alvaro ^[1] ; Simas Teixeira, Maria Francisca ^[2] ; Santos Ebinuma, Valeria de Carvalho ^[1]

Affiliation

^[1] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Bioproc Engn & Biotechnol, Araraquara, SP - Brazil

^[2] Univ Fed Amazonas, Culture Collect DPUA UFAM, BR-69077000 Manaus, Amazonas - Brazil

• Document type: Journal article
• Source: Biochemical Engineering Journal; v. 161, SEP 15 2020.
• Web of Science Citations: 0

Abstract

Fungi bioproducts have recently become commercially important to the food and pharmaceutical industry. However, different structural forms exhibited by filamentous fungi throughout their life cycles are often a bottleneck for productivity in many industrial processes. In this work, the relationship among mycelium accumulation, morphology, and colorant production was evaluated. Experiments were performed in an incubator shaker and in a stirred-tank bioreactor. First, a series of nitrogen sources and pure vitamins were investigated as additives to the monosodium glutamate-glucose medium in order to improve Talaromyces amestolkiae red colorant production in the incubator shaker. MSG and pH played an important role in generating stress, which affected cell growth and colorants production. The colorant synthesis seems to be is triggered under metabolic stress. Next, the effects of starter culture composition (MSG-glucose and nitrogen complex starter media) and airflow (2.0 and 8.0 Lmin-1()) were investigated in the bioreactor. After 120 h of cultivation in the bioreactor, a maximum red colorant production of 9.33 UA(500nm), 72.38 % glucose consumption, and 4.53 g L-1 biomass concentration were achieved under complex nitrogen as starter medium at 8 Lmin(-1). Growth remained at moderate level, probably affected by the synergistic effect of MSG and pH as stress factors. Moreover, pellets formation favored suitable oxygenation of the culture broth leading to the best consumption of the carbon source. (AU)

• FAPESP's process: 17/50303-0 - Sustainable citrus waste biorefining as a source of value added bioproducts
• Grantee: Valéria de Carvalho Santos Ebinuma
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/01580-3 - Biotechnological process for the development of new natural colorants from microorganisms for industrial application
• Grantee: Valéria de Carvalho Santos Ebinuma
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/18076-0 - Production of natural colorants by submerged cultivation of Talaromyces amestolkiae in agitated tank bioreactor by varying partial oxygen pressure and impeller type
• Grantee: Laura Carmona Ferreira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation