| Full text | |
| Author(s): |
Alcantara-de la Cruz, Ricardo
[1]
;
Amaral, Gabriel da Silva
[1]
;
de Oliveira, Guilherme Moraes
[2]
;
Rufino, Luiz Renato
[3]
;
de Azevedo, Fernando Alves
[4]
;
de Carvalho, Leonardo Bianco
[5]
;
das Gracas Fernandes da Silva, Maria Fatima
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos - Brazil
[2] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, BR-13565905 Sao Carlos - Brazil
[3] Univ Fed Sao Carlos, Ctr Ciencias Agr, Campus Araras, BR-13604900 Araras - Brazil
[4] Inst Agron Campinas IAC, Ctr Citricultura Sylvio Moreira, BR-13490970 Cordeiropolis - Brazil
[5] Sao Paulo State Univ, Sch Agr & Vet Sci, UNESP, BR-14884900 Jaboticabal - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | AGRICULTURE-BASEL; v. 10, n. 7 JUL 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Glyphosate is the main tool for weed management in Brazilian citrus orchards, where weeds, such asConyza bonariensisandDigitaria insularis, have been found with resistance to this herbicide. Field prospections have allowed the identification of a possible new case of glyphosate resistance. In this work, the susceptibility levels to glyphosate on threeAmaranthus viridisL. populations, with suspected resistance (R1, R2, and R-IAC), collected in citrus orchards from the Sao Paulo State, Brazil, as well as their accumulation rates of shikimic acid, were determined. The fresh weight of the susceptible population (S) was reduced by 50% (GR(50)) with similar to 30 g ea ha(-1)glyphosate, while the GR(50)values of the R populations were between 5.4 and 11.3 times higher than that for S population. The LD50 (herbicide dose to kill 50% of individuals of a weed population) values of the S population were <= 150 g ea ha(-1) glyphosate, while the LD(50)of the R populations ranged from 600 to 920 g ea ha(-1). Based on the reduction of fresh weight and the survival rate, the R1 population showed the highest level of glyphosate resistance, which had GR(50) and LD50 values of 248 and 918 g ea ha(-1)glyphosate, respectively. The S population accumulated 240 mu g shikimic acid at 1000 mu M glyphosate, while the R1, R2, and R-IAC populations accumulated only 16, 43, and 33 mu g shikimic acid, respectively (between 5.6 to 15 times less than the S population). Enzyme activity assays suggested that at least one target site-type mechanism was involved in resistance. This result revealed the first report of glyphosate resistance inA. viridisreported in the world. (AU) | |
| FAPESP's process: | 14/50918-7 - INCT 2014: for the Biorational Control of Pest-Insect and Phytopathogens |
| Grantee: | Maria Fátima das Graças Fernandes da Silva |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/15527-0 - Quantification and enzyme activity of the 5-enolpyruvylshikimate-3-phosphate synthase from glyphosate resistance weeds |
| Grantee: | Guilherme Moraes de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 18/15910-6 - The biorational control of PEST-INSECT and phytopathogens |
| Grantee: | Ricardo Alcántara de la Cruz |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |