| Full text | |
| Author(s): |
Camara, Guilherme A.
[1]
;
Nishiyama-Jr, Milton Y.
;
Kitano, Eduardo S.
[2]
;
Oliveira, Ursula C.
[3]
;
da Silva, Jr., Pedro I.
[3]
;
Junqueira-de-Azevedo, Inacio L.
[3]
;
Tashima, Alexandre K.
[1, 3]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Heart Inst InCor, Immunol Lab, Sao Paulo - Brazil
[3] Nishiyama-Jr, Jr., Milton Y., Inst Butantan, Ctr Toxins Immune Response & Cell Signaling, Lab Especial Toxinol Aplicada, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PHARMACOLOGY; v. 11, JUL 17 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
TheAraneaeorder is considered one of the most successful groups among venomous animals in the world. An important factor for this success is the production of venoms, a refined biological fluid rich in proteins, short peptides and cysteine-rich peptides (CRPs). These toxins may present pharmacologically relevant biological actions, as antimicrobial, antiviral and anticancer activities, for instance. Therefore, there is an increasing interest in the exploration of venom toxins for therapeutic reasons, such as drug development. However, the process of peptide sequencing and mainly the evaluation of potential biological activities of these peptides are laborious, considering the low yield of venom extraction and the high variability of toxins present in spider venoms. Here we show a robust methodology for identification, sequencing, and initial screening of potential bioactive peptides found in the venom ofAcanthoscurria rondoniae. This methodology consists in a multiomics approach involving proteomics, peptidomics and transcriptomics analyses allied toin silicopredictions of antibacterial, antifungal, antiviral, and anticancer activities. Through the application of this strategy, a total of 92,889 venom gland transcripts were assembled and 84 novel toxins were identified at the protein level, including seven short peptides and 10 fully sequenced CRPs (belonging to seven toxin families).In silicoanalysis suggests that seven CRPs families may have potential antimicrobial or antiviral activities, while two CRPs and four short peptides are potentially anticancer. Taken together, our results demonstrate an effective multiomics strategy for the discovery of new toxins andin silicoscreening of potential bioactivities. This strategy may be useful in toxin discovery, as well as in the screening of possible activities for the vast diversity of molecules produced by venomous animals. (AU) | |
| FAPESP's process: | 17/21052-0 - Sepsis: mechanisms, therapeutic targets and epidemiology |
| Grantee: | Reinaldo Salomão |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/50040-4 - Rational approach for searching molecular targets involved in inflammatory events and cell survival |
| Grantee: | Ana Marisa Chudzinski-Tavassi |
| Support Opportunities: | Research Grants - Research Centers in Engineering Program |