Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immobilization of Paclitaxel on Hydroxyapatite for Breast Cancer Investigations

Full text
Author(s):
Martins, Murillo L. [1] ; Pinto, Thais S. [2] ; Gomes, Anderson M. [2] ; Parra, Joao P. R. L. L. [2] ; Franchi, Jr., Gilberto C. [3] ; Zambuzzi, Willian F. [2] ; Rodrigues, Cloves G. [1, 4]
Total Authors: 7
Affiliation:
[1] Pontifical Catholic Univ Goias, Postgrad Program Ind & Syst Engn, BR-74175120 Goiania, Go - Brazil
[2] State Univ Sao Paulo UNESP, Biosci Inst Botucatu IBB, Chem & Biochem Dept, Lab Bioassays & Cellular Dynam LaBIO, BR-18618000 Botucatu, SP - Brazil
[3] Univ Campinas UNICAMP, Fac Med Sci, Oncohematol Child Res Ctr CIPOI, BR-13083970 Campinas, SP - Brazil
[4] Pontifical Catholic Univ Goias, Sch Exact Sci & Comp, BR-74175120 Goiania, Go - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Langmuir; v. 36, n. 30, p. 8723-8732, AUG 4 2020.
Web of Science Citations: 0
Abstract

A simple method for immobilization of the chemotherapy drug paclitaxel (PTX) on hydroxyapatite nanoparticles (n-HAP) using the biopolymer chitosan as a trapping agent is described focusing on applications involving breast cancer cells. n-HAP with two distinct crystallinity profiles were used: with predominant crystallization along the long axis and with a more homogeneous crystallization in all directions. In the first scenario, the interactions between chitosan and both the OH and PO43- groups on the surface of the nanoparticles are favored and lead to a more efficient attachment of the drug. In this case, PTX is found to remain mostly attached to the n-HAP for at least 24 h, while being dispersed in aqueous solution. During this time, the activity of the drug is inhibited as corroborated by in vitro assays with breast cancer cells. With that, the in vitro experiments revealed distinct effects from the drug-loaded nanoparticles on the cells depending on the experimental conditions. In a short term, that is, in 24 h, the cells exhibit higher viability than those challenged with nonloaded materials. Nevertheless, after 72 h, even a small content of PTX in the presence of n-HAP can reduce the cells' viability via stimulation of the apoptotic phenotype and suppression of survival stimuli. (AU)

FAPESP's process: 19/09735-0 - BONEInk: synthesis and characterization of chitosan crosslinked with carbon nanotubes modified with RGD peptides and HA crystals for potential application of bioimpression of bone tissue
Grantee:João Paulo Ruiz Lucio de Lima Parra
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Research Grants - Young Investigators Grants