Effects of different equilibration times at 5 degrees C on boar sperm cryotolerance

Author(s):Show less -

Passarelli, Marina da Silva ^[1] ; Pinoti Pavaneli, Ana Paula ^[1] ; Ravagnani, Gisele Mouro ^[1] ; Martins, Matheus Pasini ^[1] ; Pedrosa, Ana Carolina ^[1] ; Massami Kitamura Martins, Simone Maria ^[2] ; de Alcantra Rocha, Nathalia Raissa ^[3] ; Bittar Rigo, Victor Henrique ^[1] ; Yasui, George ^[3] ; Yeste, Marc ^[4] ; Cesar de Andrade, Andre Furugen ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Androl & Technol Swine Embryos, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga, SP - Brazil

^[3] Lab Biotechnol Fishs CEPTA ICMBio, Pirassununga, SP - Brazil

^[4] Univ Girona, Biotechnol Anim & Human Reprod TechnoSperm, Unit Cell Biol, Dept Biol, Inst Food & Agr Technol, Fac Sci, Girona - Spain

• Document type: Journal article
• Source: Animal Reproduction Science; v. 219, AUG 2020.
• Web of Science Citations: 1

Abstract

Equilibration time (ET) is the period during which sperm cells are in contact with cooling/freezing media components at a temperature of 5 degrees C, providing a proper osmotic balance between the intra- and extra-cellular milieu. The present study aimed to determine the ET (0, 2, and 4 h) that results in greater post-thaw sperm quality and functions. Based on the post-thaw sperm membrane integrity and motility ratios, 20 ejaculates collected from five boars were classified as having good (GFE, n = 5) or poor (PFE, n = 15) freezing capacity. Ratios of post-thaw sperm with intact plasma membrane and acrosome were similar between ET (0 h: 37.02 % +/- 2.85 %; 2 h: 34.59 % +/- 7.12 %; 4 h: 37.87 % +/- 4.44 %) in GFE samples. In PFE, ratios of sperm with intact plasma membrane and acrosome at post-thaw were greater (P < 0.05) after an ET of 2 h than after an ET of 0 h (2 h: 26.16 % +/- 1.54 % and 0 h: 16.74 % +/- 1.59 %). Also, ratios of post-thaw sperm with relatively lesser membrane lipids disorder were greater (P < 0.05) after an ET of 2 h than for other ET in both GFE (2 h: 21.97 % +/- 4.24 % and 0 h: 16.63 % +/- 2.38 %) and PFE (2 h: 16.65 % +/- 1.40 % and 0 h: 13.23 % +/- 1.25 %) samples. In conclusion, an ET of 2 h results in greater sperm cryotolerance in both GFE and PFE samples, which suggests that modifying the freezing protocol lead to an increase post-thaw sperm function and survival. (AU)

• FAPESP's process: 16/24690-4 - Metabolomics signatures on the influence of holding time on increasing boar spermatozoa cryotolerance
• Grantee: André Furugen Cesar de Andrade
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/01124-1 - Holding time and equilibration time: Its effects on cryopreserved boar spermatozoa
• Grantee: Marina da Silva Passarelli Zanatta
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 17/10821-2 - Evaluation of equilibration time on boar semen cryopreservation protocols: a physiological and morphofunctional study
• Grantee: Marina da Silva Passarelli Zanatta
• Support Opportunities: Scholarships in Brazil - Master