The sugarcane ShMYB78 transcription factor activates suberin biosynthesis inNicotiana benthamiana

Author(s)

Figueiredo, Raquel ^{[1, 2]} ; Portilla Llerena, Juan Pablo ^[2] ; Kiyota, Eduardo ^[2] ; Ferreira, Savio Siqueira ^[3] ; Cardeli, Barbara Rocha ^[2] ; Ribeiro de Souza, Sarah Caroline ^{[4, 2]} ; Brito, Michael dos Santos ^[5] ; Sodek, Ladaslav ^[2] ; Cesarino, Igor ^[3] ; Mazzafera, Paulo ^{[2, 6]}

Affiliation

^[1] Univ Porto, Fac Ciencias, Dept Biol, Rua Campo Alegre S-N, P-4169007 Porto - Portugal

^[2] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, BR-13083862 Campinas - Brazil

^[3] Univ Sao Paulo, Inst Biosci, Dept Bot, BR-05508090 Sao Paulo - Brazil

^[4] Univ Fed Sao Carlos, Dept Bot, POB 676, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Fed Sao Paulo, Inst Sci & Technol, Campus Sao Jose dos Campos, BR-12231280 Sao Jose Dos Campos, SP - Brazil

^[6] Univ Sao Paulo, Coll Agr Luiz Queiroz, Dept Crop Sci, BR-13418900 Piracicaba - Brazil

• Document type: Journal article
• Source: Plant Molecular Biology; v. 104, n. 4-5 AUG 2020.
• Web of Science Citations: 0

Abstract

Key message A sugarcane MYB present in the culm induces suberin biosynthesis and is involved both with fatty acid and phenolics metabolism. Few transcription factors have been described as regulators of cell wall polymers deposition in C4 grasses. Particularly, regulation of suberin biosynthesis in this group of plants remains poorly understood. Here, we showed that the sugarcane MYB transcription factor ShMYB78 is an activator of suberin biosynthesis and deposition. ShMYB78 was identified upon screening genes whose expression was upregulated in sugarcane internodes undergoing suberization during culm development or triggered by wounding.Agrobacterium-mediated transient expression ofShMYB78inNicotiana benthamianaleaves induced the ectopic deposition of suberin and its aliphatic and aromatic monomers. Further, the expression of suberin-related genes was induced byShMYB78heterologous expression inNicotiana benthamianaleaves. ShMYB78 was shown to be a nuclear protein based on its presence in sugarcane internode nuclear protein extracts, and protoplast transactivation assays demonstrated that ShMYB78 activates the promoters of the sugarcane suberin biosynthetic genes beta-ketoacyl-CoA synthase (ShKCS20) and caffeic acid-O-methyltransferase (ShCOMT). Our results suggest that ShMYB78 may be involved in the transcriptional regulation of suberin deposition, from fatty acid metabolism to phenylpropanoid biosynthesis, in sugarcane internodes. (AU)

• FAPESP's process: 15/02527-1 - Development of model systems in sorghum and a systems biology approach to unravel the molecular mechanisms controlling lignin metabolism in grasses
• Grantee: Igor Cesarino
• Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• FAPESP's process: 15/05437-3 - Suberin biopolimer and the digestibility of sugarcane cell wall: histochemistry, biochemistry and molecular studies
• Grantee: Ana Raquel dos Santos Figueiredo
• Support type: Scholarships in Brazil - Post-Doctorate