Probiotics alter biofilm formation and the transcription ofPorphyromonas gingivalisvirulence-associated genes

Author(s)

Ishikawa, Karin Hitomi ^[1] ; Mita, Daniela ^[1] ; Kawamoto, Dione ^[1] ; Nicoli, Jacques Robert ^[2] ; Albuquerque-Souza, Emmanuel ^{[1, 3]} ; Lorenzetti Simionato, Maria Regina ^[1] ; Alves Mayer, Marcia Pinto ^{[1, 3]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[2] Univ Fed Minas Gerais, Biol Sci Inst, Dept Microbiol, Belo Horizonte, MG - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Stomatol, Div Periodont, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF ORAL MICROBIOLOGY; v. 12, n. 1 JAN 1 2020.
• Web of Science Citations: 0

Abstract

Background and Objective The potential of probiotics on the prevention and control of periodontitis and other chronic inflammatory conditions has been suggested.LactobacillusandBifidobacteriumspecies influenceP. gingivalisinteraction with gingival epithelial cells (GECs) but may not act in a unique way. In order to select the most appropriate probiotic againstP. gingivalis, we aimed to evaluate the effect of several strains onPorphyromonas gingivalisbiofilm formation and transcription virulence-associated factors (PgVAFs). Methods Cell-free pH neutralized supernatants (CFS) and livingLactobacillusspp. andBifidobacteriumspp. were tested againstP. gingivalisATCC 33277 and W83, in mono- and multi-species (withStreptococcus oralisandS. gordonii) biofilms. Relative transcription ofP. gingivalisgenes (fimA, mfa1, kgp, rgp, ftsHandluxS) was determined in biofilms and under GECs co-infection. Results Probiotics CFS reducedP. gingivalisATCC 33277 levels in mono-species biofilms and living probiotics reducedP. gingivalisabundance in multi-species biofilms.L. acidophilusLA5 down-regulated transcription of most PgVAFs in biofilms and GECs. Conclusions Probiotics affectP. gingivalisbiofilm formation by down-regulating overall PgVAFs with the most pronounced effect observed forL. acidophilusLA5. (AU)

• FAPESP's process: 16/13156-7 - Direct and immunomodulatory effects of probiotic bacteria on periodontal pathogens
• Grantee: Karin Hitomi Ishikawa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/18273-9 - New strategies for the control of periodontitis
• Grantee: Marcia Pinto Alves Mayer
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/14687-6 - Evaluation of immunomodulatory potential of probiotics in periodontal disease
• Grantee: Emmanuel Albuquerque de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/13159-6 - Supra and subgingival microbiota diversity analysis and protein profile of saliva and enamel film of individuals with chronic periodontitis and microbial succession
• Grantee: Dione Kawamoto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral